Implementation and application of FRET-FLIM technology  

作　　者：Shiqi Wang Binglin Shen Sheng Ren Yihua Zhao Silu Zhang Junle Qu Liwei Liu 

机构地区：[1]Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University,Shenzhen,518060,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2019年第5期57-68,共12页创新光学健康科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(61722508/61525503/61620106016/81727804);The National Key Research and Development Program of China(2017YFA0700402);Guangdong Natural Science Foundation Innovation Team(2014A030312008);Shenzhen Basic Research Project(JCYJ20150930104948169/JCYJ20160328144746940/GJHZ20160226202139185/JCYJ20170412105003520)。

摘　　要：With the development of the new detection methods and the function of fluorescent molecule,researchers hope to further explore the internal mechanisms of organisms,monitor changes in the intracellular microenvironment,and dynamic processes of molecular interactions in cells.Fluo-rescence resonance energy transfer(FRET)describes the energy transfer process between donor fluorescent molecules and acceptor fluorescent molecules.It is an important means to detect protein-protein interactions and protein conformation changes in cells.Fluorescence lifetime imaging microscopy(FLIM)enables noninvasive measurement of the fAuorescence lifetime of fluorescent particles in vivo.The FRET-FLIM technology,which is use FLIM to quantify and analyze FRET,enables real-time monitoring of dynamic changes of proteins in biological cells and analysis of protein interaction mechanisms.The distance between donor and acceptor and their respective fAuorescent lifetime,which are of great importance for studying the mechanism of intracellular activity can be obtained by data analysis and algorithm ftting.

关 键 词：Fluorescence resonance energy transfer fuorescenc-lifetime imaging microscopy protein-protein interaction 

分 类 号：O62[理学—有机化学]