A ggregation-induced emission nanoparticles for in vivo three-photon fuorescence microscopic rat brain angiography  

作　　者：Hequn Zhang Weisi Xie Ming Chen Liang Zhu Zhe Feng Yalun Wang Wang Xi Ben Zhong Tang Jun Qian 

机构地区：[1]Centre for Optical and Electromagnetic Research Zhejiang University,Hangzhou 310058,P.R.China [2]Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction Division of Life Science,State Key Laboratory of Molecular Neuroscience Institute for Advanced Study,Institute of Molecular Functional Materials Division of Biomedical Engineering The Hong Kong University of Science and Technology Clear Water Bay,Kowloon,Hong Kong,P.R.China [3]Interdisciplinary Institute of Neuroscience and Technology(ZIINT)Zhejiang University,Hangzhou,310058,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2019年第6期34-45,共12页创新光学健康科学杂志（英文）

基　　金：supported by the Zhejiang Provincial Natural Science Foundation of China(LR17F050001 and LY17C090005);the National Natural Science Foundation of China(61735016 and 91632105);National Basic Research Program of China(973 Program,2013CB834701 and 2013CB834704).

摘　　要：Rodents are popular biological models for physiological and behavioral research in neuroscience and rats are better models than mice due to their higher genome similarity to human and more accessible surgical procedures.However,rat brain is larger than mice brain and it needs powerful imaging tools to implement better penetration against the scattering of the thicker brain tissue.Three-photon fluorescence microscopy(3PFM)combined with near-infrared(NIR)excitation has great potentials for brain circuits imaging beause of its abilities of anti scattering,deep-tissue imaging,and high signal-to-noise ratio(SNR).In this work,a type of AIE lumninogen with red fuorescence was synthesized and encapsulated with Pluronic F-127 to make up form nano-particles(NPs).Bright DCDPP-2TPA NPs were employed for in trino three-photon fuorescent laser scanning microscopy of blood vessels in rats brain under 1550 nm femtosecond laser exci-tation.A fine three-dimensional(3D)reconstruction up to the deepness of 600 pm was achieved and the blood flow velocity of a selected vessel was measured in vrito as well.Our 3PFM deep brain imaging method simultaneously recorded the morphology and function of the brain blood vessels in vivo in the rat model.Using this angiography combined with the arsenal of rodent's brain disease,models can accelerate the neuroscience research and clinical diagnosis of brain disease in the future.

关 键 词：Three photon fluorescence microscopy(3PFM) aggregation induced emision(AIE) deep-tissue imaging in vivo rat brain 

分 类 号：TH742[机械工程—光学工程]