Optimization method to suppress background for imaging multiple planes  

作　　者：Vannhu Le 

机构地区：[1]Department of Optical Engineering Le Quy Don Technical University,236 Hoang Quoc Viet Street Tu Liem District,Hanoi,Vietnam [2]State Key Laboratory of Modern Optical Instrumentation College of Optical Science and Engineering Zhejiang University,Hangzhou 310027,P.R.China [3]Institute of Research and Development Duy Tan University,Da Nang 550000,Vietnam

出　　处：《Journal of Innovative Optical Health Sciences》2019年第2期24-34,共11页创新光学健康科学杂志（英文）

基　　金：This work is supported by the Vietnam National Foundation for Science and Technology Development(NAFOSTED)under Grant number(103.03-2018.08).

摘　　要：3D live imaging is important for better understanding of biological processes.To obtain biological dynamic process,high imaging speed is required.In order to improve speed in 3D live imaging,simultaneous imaging of multiple planes throughout a 3D volume has been proposed.However,a main disadvantage of this method is the cross-talk from neighboring imaging planes.In this paper,we propose an optimization method to suppress background from neighboring imaging planes.A D-aperture is used to generate multiple light sheets.An optimization method to suppress background is presented.The simulation results demonstrated that the proposed method can be used to suppress the effectiveness of background from neighboring light sheets.

关 键 词：Fluorescence microscopy imaging systems supper-resolution 

分 类 号：R318[医药卫生—生物医学工程]