骨骼肌钝挫伤对细胞铁代谢的影响和铁死亡相关性研究  

Effect of Skeletal Muscle Contusion on Cellular Iron Metabolism and its Correlation with Ferroptosis

作　　者：李家林[1] 储丽琴[1] 唐志家 施炜[1] 唐吉斌[2] 邢益祥 LI Jian-lin;CHU Li-qin;TANG Zhi-jia;SHI Wei;TANG Ji-bin;XING Yi-xiang(Tongling Polytechnic,Anhui Tongling 244061,China;Tongling People's Hospital,Anbui,Tongling,244009,China)

机构地区：[1]铜陵职业技术学院,安徽铜陵244061 [2]铜陵市人民医院

出　　处：《承德医学院学报》2023年第1期11-16,共6页Journal of Chengde Medical University

基　　金：安徽省高等学校省级质量工程项目(2018jxtd122);铜陵职业技术学院科学研究重点项目(tlpt2020NK007)。

摘　　要：目的 探讨大鼠骨骼肌钝挫伤后对细胞铁代谢的影响及其与铁死亡的相关性。方法 SD雄性大鼠,随机分为对照组和钝挫伤模型组,模型组分别于伤后1d、3d、5d、7d、14d取材。HE染色观察损伤组织病理变化;普鲁士蓝染色观察损伤区铁离子沉积情况;ELISA法检测血清铁蛋白和肌组织ROS、α-KG及HSPB1含量变化。结果HE染色显示挫伤区肌细胞膜破裂,核溶解,炎性细胞浸润,5d后开始减轻,14d挫伤区新生骨骼肌细胞进一步增多,细胞间隙胶原纤维增多。普鲁士蓝染色显示挫伤后铁离子沉积渐增高,5d组最高,5d后下降。ELISA结果显示,模型组血清铁蛋白、ROS、α-KG在损伤后均逐渐升高,与对照组比较差异有统计学意义(P<0.01),HSPB1在损伤后先升高后降低,与对照组比较差异有统计学意义(P<0.01)。结论 骨骼肌钝挫伤后铁代谢异常与铁死亡发生密切相关,骨骼肌急性损伤后也存在细胞铁死亡。Objective To investigate the effect of skeletal muscle contusion on iron metabolism and its correlation with ferroptosis in rats. Methods Male SD rats were randomly divided into control group and blunt contusion model group. The model group was selected at 1d, 3d, 5d, 7d and 14d after injury, respectively. HE staining was used to observe the pathological changes of the injured tissue, and Prussian blue staining was used to observe the iron ion deposition in the injured area. ELISA method was used to detect the changes of serum ferritin, muscle tissue reactive oxygen species, α-ketoglutarate and heat shock protein 1. Results HE staining showed the rupture of muscle membrane, nucleolysis and inflammatory cell infiltration in the contusion area, which began to decrease after 5 days. The number of new skeletal muscle cells in the contusion area increased further on 14 days, and the number of intercellular collagen fibers increased. Prussian blue staining showed that iron deposition increased gradually after contusion, the highest in fifth day group, and then decreased. ELISA results showed that serum ferritin, ROS and α-KG in the model group increased gradually after injury, and the difference was statistically significant compared with the control group(P<0.01). HSPB1 first increased and then decreased after injury, and the difference was statistically significant compared with the control group(P<0.01). Conclusion Abnormal iron metabolism is closely related to ferroptosisafter skeletal muscle contusion, and cell ferroptosis also occurs after skeletal muscle acute injury.

关 键 词：骨骼肌钝挫伤 铁代谢 氧化应激 铁死亡 

分 类 号：R34[医药卫生—基础医学]