ω-3多不饱和脂肪酸对热应激小鼠肝脏损伤的保护作用研究  

Protective Effect of Omega-3 Polyunsaturated Fatty Acid on Hepatic Damage Induced by Heat Stress in Mice

作　　者：王亚娜 杨慧娣 李光鹏 WANG Yana;YANG Huidi;LI Guangpeng(Basic Medical College of Inner Mongolia Medical University,Inner Mongolia 010110,China)

机构地区：[1]内蒙古医科大学基础医学院,呼和浩特010110 [2]内蒙古大学省部共建草原家畜生殖调控与繁育国家重点实验室,呼和浩特010070

出　　处：《医学研究杂志》2023年第1期37-41,51,共6页Journal of Medical Research

基　　金：国家自然科学基金资助项目(32160247);内蒙古自治区自然科学基金资助项目(2020MS03059)。

摘　　要：目的探讨ω-3多不饱和脂肪酸(omega-3 polyunsaturated fatty acid,ω-3 PUFA)对热应激小鼠肝脏损伤的影响。方法成年雄性健康野生型C57BL/6C小鼠16只和FAD3转基因小鼠16只分为C57BL/6C组(n=8)、C57BL/6H组(n=8)、FAD3C组(n=8)和FAD3H组(n=8)。C57BL/6C组和FAD3C组给予室温环境(23±1℃),C57BL/6H组和FAD3H组给予高温处理(30℃),自由取食饮水,每4天记录1次小鼠体质量和直肠温度,第7周末,取血后处死小鼠并取其肝脏组织,称重并记录肝重。计算平均体质量增量、平均日摄食量以及肝重比(肝重/终体质量)。采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)和丙氨酸氨基转移酶(alanine aminotransferase,ALT)的含量,以评估小鼠肝脏损伤的状况。实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)检测肝脏中肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-10(interleukin-10,IL-10)、单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)以及热休克蛋白72(heat shock proteins 72,HSP72)mRNA表达水平。结果与C57BL/6C组比较,C57BL/6H组小鼠肝脏HSP72、TNF-α、IL-1β、IL-6、MCP-1mRNA表达水平以及血清AST、ALT含量均显著增加(P<0.05);然而,与C57BL/6H组比较,FAD3H组小鼠肝脏HSP72、TNF-α、IL-1β、IL-6、MCP-1mRNA表达水平以及血清AST、ALT含量均显著降低(P<0.05)。结论ω-3 PUFA通过抑制高温诱导的热应激小鼠肝脏中促炎性细胞因子TNF-α、IL-1β、IL-6以及MCP-1mRNA的表达水平,降低血清AST和ALT的含量,下调HSP72 mRNA表达水平,进而缓解热应激小鼠肝脏的损伤。Objective To explore the effect of omega-3 polyunsaturated fatty acid(ω-3 PUFA)on hepatic damage induced by heat stress in mice.Methods 16 adult male healthy C57BL/6C wild-type mice and 16 FAD3 transgenic mice were divided into four groups:C57BL/6C group(n=8),C57BL/6H group(n=8),FAD3C group(n=8)and FAD3H group(n=8).The mice of the C57BL/6C group and FAD3C group were given room temperature(23±1℃),and mice of the C57BL/6H group and FAD3H group were given high temperature(30℃)intervention with free access to food and water.The body weight and rectal temperature of the mice were recorded every 4days.At the end of the 7th week,the mice were sacrificed after blood collection and liver tissues were collected,weighed and liver weight was recorded.Average weight gain,average daily feed intake,and liver weight ratio(liver weight/final body weight)were calculated.The serum aspartate aminotransferase(AST)and alanine aminotransferase(ALT)were detected by the enzyme linked immunosorbent assay(ELISA),which were used to evaluate the status of hepatic injury in mice.The mRNA expression level of tumor necrosis factor-alpha(TNF-α),interleukin-1 beta(IL-1β),interleukin-6(IL-6),interleukin-10(IL-10),monocyte chemoattractant protein-1(MCP-1),and heat shock proteins 72(HSP72)were detected by real-time quantitative polymerase chain reaction(RT-qPCR).Results The HSP72,TNF-α,IL-1β,IL-6 and MCP-1mRNA expression levels of the liver and serum AST and ALT levels in the C57BL/6H group were significantly higher than those in the C57BL/6C group(P<0.05).However,The hepatic HSP72,TNF-α,IL-1β,IL-6 and MCP-1mRNA expression levels of the liver as well as serum AST and ALT levels in the FAD3H group were significantly lower than those in the C57BL/6C group(P<0.05).Conclusionω-3 PUFA can reduce the mRNA expression level of TNF-α,IL-1β,IL-6,MCP-1,decrease the content of AST and ALT in serum,and down-regulate the mRNA expression level of HSP70 in the liver of mice induced by heat stress,so as to alleviate the hepatic damage induced by heat

关 键 词：热应激 热休克蛋白72 炎症 Ω-3多不饱和脂肪酸 

分 类 号：R339[医药卫生—人体生理学]