藏药五味姜黄膏药效学及急性毒性分析  

Analysis and evaluation of pharmacodynamics and acute toxicity of Tibetan medicine Wuwei Jianghuang ointment

作　　者：丹增次珍 普布扎西 朱星昊 陈计智 吴宗耀 Danzengcizhen;Pubuzhaxi;ZHU Xinghao;CHEN Jizhi;WU Zongyao(Institute of Tibetan Medicine,Tibet University of Tibetan Medicine,Lhasa 850000,China;Department of Pharmacy,Tibetan Hospital of Tibet Autonomous Region,Lhasa 850000,China;Monitoring and Statistical Research Center,National Administration of Traditional Chinese Medicine,Beijing 100027,China)

机构地区：[1]西藏藏医药大学藏医药研究所,西藏拉萨850000 [2]西藏自治区藏医院药剂科,西藏拉萨850000 [3]国家中医药管理局监测统计中心,北京100027

出　　处：《医学研究与教育》2022年第6期1-8,共8页Medical Research and Education

基　　金：西藏自治区科技计划重点研发计划项目(XZ202001ZY0049N);2020年中医学(藏医)博士点建设科研支撑计划项目(BSDJS-20-19)。

摘　　要：目的挖掘藏医治疗朗秀病(湿疹)的经典方药,并对应用广、疗效好的名方五味姜黄膏的经典理论进行梳理完善,考证其对湿疹小鼠模型的干预作用,分析其对家兔皮肤的急性毒性影响。方法取BALB/c小鼠50只,随机选取10只作为空白组,其余40只制备小鼠湿疹模型。用7%高浓度DNCB丙酮橄榄油溶液致敏2次,再用0.5%低浓度DNCB丙酮橄榄油溶液20μL激发1次,观察小鼠背部皮肤出现红斑、水肿及渗出液等急性皮炎现象。造模成功后,将40只湿疹小鼠按随机数字表法分成4组,分别是模型组、丁酸氢化可的松乳膏组、五味姜黄膏高剂量组、五味姜黄膏低剂量组,每组10只。除模型组外,其余3组分别给予相应药物外涂干预36 d,确定药物疗效。另取新西兰家兔16只,随机数字表法将其分为4组,造模,涂药。连续观察14 d,每日观察2次,观察其安全性。结果对各组小鼠背部皮肤采用盲法计分,五味姜黄膏高、低剂量对小鼠背部皮损组织有良好的愈合作用。与模型组比较,五味姜黄膏高、低剂量和丁酸氢化可的松乳膏在降低第15、30天皮损积分(P<0.01)、降低小鼠耳肿胀厚度(P<0.01)、降低小鼠脾脏指数(P<0.01)方面,差异有统计学意义。与模型组比较,五味姜黄膏高、低剂量和丁酸氢化可的松乳膏在降低小鼠血清TNF-α水平方面,差异有统计学意义(P<0.01)。五味姜黄膏高、低剂量可降低小鼠血清IL-6,但差异无统计学意义(P>0.05)。家兔毒性实验结果表明,五味姜黄膏对家兔局部皮肤无明显损伤。结论在对比五味姜黄膏与丁酸氢化可的松乳膏阳性对照研究后,明确了五味姜黄膏治疗朗秀病(湿疹)疗效仅次于丁酸氢化可的松乳膏,证实了藏医古籍中五味姜黄膏对朗秀病(湿疹)的治疗作用。五味姜黄膏对正常皮肤、破损皮肤都可以按正常剂量使用。Objective To explore the classic prescriptions of Tibetan medicine for the treatment of Langxiu disease(eczema),and sort out and improve the classic theory of the famous prescription Wuwei Jianghuang ointment which is widely used and has good curative effects,and examines its effects on the eczema mouse model,and analyzes and evaluates its effects on the eczema mouse model,then acute toxic effects on the skin.Methods Fifty BALB/c mice,10 mice were randomly selected as blank group,and the other 40 mice were prepared to model eczema.The mice were sensitized twice with 7%high concentration DNCB acetone olive oil solution,and then stimulated once with 0.5%low concentration DNCB acetone olive oil solution 20μL.Acute dermatitis such as erythema,edema and exudation of back skin were observed.After successful modeling,40 eczema mice were randomly divided into 4 groups,which were model group,the hydrocortisone butyrate cream group Wuwei Jianghuang ointment high-dose group and Wuwei Jianghuang ointment low-dose group,with 10 mice in each group.Except the model group,the other 3 groups were treated with corresponding drugs for 36 days,respectively,to determine the drug efficacy.Another 16 New Zealand rabbits were selected and divided into 4 groups by random number table method.Continuous observation went on for 14d,2 times a day.Results Blind scoring of the back skin of mice in each group found that the high and low dose groups of Wuwei Jianghuang ointment had a good healing effect on the back skin lesions of mice.Compared with the model group,the high-dose and low-dose Wuwei Jianghuang ointment groups and the hydrocortisone butyrate cream group had statistical significance in the skin lesion scores on the 15th and 30th days(P<0.01),and had statistical significance in the ear swelling thickness(P<0.01).And had statistical significance in the spleen index of mice(P<0.01).Compared with the model group,the high and lowdose groups of Wuwei Jianghuang ointment and hydrocortisone butyrate cream groups had statistical significanc

关 键 词：藏医朗秀病 五味姜黄膏 小鼠湿疹模型 丁酸氢化可的松 DNCB致敏 

分 类 号：R285.5[医药卫生—中药学]