Elucidating the Molecular Mechanism of Dynamic Photodamage of Photosystem II Membrane Protein Complex by Integrated Proteomics Strategy  

作　　者：Ye Zhou Zheyi Liu Mingdong Yao Jun Chen Yanan Xiao Guangye Han Jian-Ren Shen Fangjun Wang 

机构地区：[1]CAS Key Laboratory of Separation Sciences for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023 [2]State Key Laboratory of Catalysis,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023 [3]Key Laboratory of Systems Bioengineering,Ministry of Education,Tianjin University,Tianjin 300072 [4]Science and Technology on Surface Physics and Chemistry Laboratory,Jiangyou 621908 [5]University of Chinese Academy of Sciences,Beijing 100049 [6]Photosynthesis Research Center,Key Laboratory of Photobiology,Institute of Botany,Chinese Academy of Sciences,Beijing 100093 [7]Research Institute for Interdisciplinary Science,Graduate School of Natural Science and Technology,Okayama University,Okayama 700-8530

出　　处：《CCS Chemistry》2022年第1期182-193,共12页中国化学会会刊（英文）

基　　金：The authors gratefully acknowledged the financial supports from the National Key R&D Program of China(nos.2016YFF0200504,2017YFA0503700,and 2018YFA0900702);the National Natural Science Foundation of China(nos.91853101 and 31470339);grants from the Chinese Academy of Sciences(nos.ZDBS-LYSLH032 and XDB17000000);the Liaoning Province(no.2019-YQ-07);the Dalian Institute of Chemical Physics(DICP;no.DICP I202007).

摘　　要：Photosystem II(PSII),as a multiple-subunit chloroplast membrane-associated pigment-protein complex on the thylakoid membrane,is a primary target of light-induced photodamage.However,the overall molecular details of the conformation and composition dynamics of PSII photodamage are still controversial.In this study,we investigated systematically the dynamic conformation,degradation,and oxidation processes of PSII photodamage by integrating chemical cross-linking and top-down proteomics strategies.

关 键 词：photosystem II dynamic photodamage chemical cross-linking top-down proteomics mass spectrometry 

分 类 号：Q51[生物学—生物化学]