Overexpression of the PdpapERF109 gene enhances resistance of Populus davidiana×P.alba var.pyramidalis to Fusarium oxysporum infection  

作　　者：Jian Diao Mingrui Li Ping Zhang Cheng Zong Wei Ma Ling Ma 

机构地区：[1]College of Forestry,Northeast Forestry University,Harbin,People’s Republic of China [2]College of Wildlife and Protected Area,Northeast Forestry University,Harbin,People’s Republic of China [3]College of Medicine,Heilongjiang University of Chinese Medicine,Harbin,People’s Republic of China

出　　处：《Journal of Forestry Research》2022年第6期1925-1937,共13页林业研究（英文版）

基　　金：supported by the Central University Basic Research Business Expenses Special Fund Project[grant number:2572018AA37];the Fundamental Research Funds for the Central Universities[2572019CP01]。

摘　　要：The key transcription factor gene PdP apE RF109 was cloned from Populus davidiana×P.alba var.pyramidalis(Pdpap),and after overexpression of P dP ap ERF109 in transformants,the gene functions in the resistance response to Fusarium oxysporum infection.Compared with the wild Pdpap,after inoculation with F.oxysporum,the physiological and biochemical characteristics,including relative fresh weight,peroxidase activity,and the percentage of electrolyte leakage showed that,after overexpression of the PdPapERF109 gene,the transformants grew well and displayed significant resistance to F.oxysporum infection.By comparing the reactive oxygen species scavenging capacity of Pdpap plants after pathogen infection,the P dPapERF109-overexpressing plants had significantly better reactive oxygen species scavenging ability than the wild plants.Comprehensive analysis of plant morphology and various physiological and biochemical parameters showed that the overexpression of the P dpapERF109 gene significantly improved the resistance of Pdpap plants to F.oxysporum root rot.Therefore,increasing the expression of the homologous ERF109 gene can be an effective strategy to increase disease resistance in hybrid poplars.

关 键 词：ERF Fusarium oxysporumtolerance Gene over-expression Genetic transformation Transcription factor 

分 类 号：S763.7[农业科学—森林保护学]