82份苹果属资源SFB基因克隆及不同生态居群基因频率分析  

Isolation of SFB Genes of 82 Malus Germplasm Resources and Gene Frequency Analysis of Different Ecological Populations

作　　者：刘昭 高源[1] 王昆[1] 冯建荣[2] 孙思邈 路翔 李青山 李连文[1] 王大江[1] LIU Zhao;GAO Yuan;WANG Kun;FENG Jian-rong;SUN Si-miao;LU Xiang;LI Qing-shan;LI Lian-wen;WANG Da-jiang(Research Institute of Pomology,Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Germplasm Resources Utilization,Ministry of Agriculture and Rural Affairs,Liaoning Xingcheng 125100;College of Agriculture,Shihezi University/Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization,Xinjiang Shihezi 832000)

机构地区：[1]中国农业科学院果树研究所/农业农村部园艺作物种质资源利用重点实验室,辽宁兴城125100 [2]石河子大学农学院/特色果树栽培生理与种质资源利用兵团重点实验室,新疆石河子832000

出　　处：《植物遗传资源学报》2023年第1期215-225,共11页Journal of Plant Genetic Resources

基　　金：中国农业科学院创新工程项目(CAAS-ASTIP-2021-RIP-02)。

摘　　要：为了明确不同苹果属资源的SFB基因及在各居群中SFB基因的类型和分布频率,本研究以奎花等栽培苹果及新疆野苹果等野生资源共82份为试验材料,利用3对苹果属SFB基因特异性引物,以叶片DNA为模板进行PCR扩增。特异扩增结果显示:所有试材中均只鉴定出单一的SFB基因片段。经GenBank同源性比较分析,发现这些片段分属5种不同的SFB等位基因,分别为SFB1、SFB2、SFB3、SFB7、SFB9。同时对不同生态种群中SFB基因的分布频率进行了分析,结果表明不同SFB基因出现的频率不同,SFB3出现的频率最高为43.90%,其次为SFB1(39.00%),而相同的SFB基因在不同苹果种间及种内出现的频率亦不同。此外,系统进化树分析显示,新疆野苹果与土库曼苹果及栽培苹果的驯化起源有关。In order to clarify the SFB gene diversity in Malus Mill. and the distribution frequency of each sequence haplotype in ecological populations, 82 Malus Mill. germplasm resources including the cultivated apples(i.e. Kuihua) and wild resources(i.e. Malus sieversii(Ledeb.) M. Roem.) were used. The genotyping was conducted using three gene-specific primer combinations. PCR amplification using leaf DNA produced a single SFB gene fragment in all materials. Sanger sequencing of PCR products revealed these fragments belonged to five different SFB genes, corresponding SFB1, SFB2, SFB3, SFB7and SFB9based on GenBank homology comparison. At the same time, the distribution frequency of SFB gene in different ecological population was analyzed. The frequencies of different haplotypes in populations were different, and the highest frequency of SFB3was 43.90%, followed by SFB1(39.00%). The intraspecific and interspecific frequency of the same SFB gene was also different. In addition, the phylogenetic tree analysis showed that Malus sieversii(Ledeb.) M.Roem. was related to the domestication and origin of Malus turkmenorumJuz. & M. Pop. and apple cultivars.

关 键 词：苹果属 种质资源 SFB基因 克隆 频率 

分 类 号：S661.1[农业科学—果树学]