低温胁迫下四倍体黄肉中华猕猴桃转录组分析  

Transcriptome Analysis of Cold-treated Tetraploid Yellow-fleshed Actinidia chinensis Planch.

作　　者：杜朝金 李贻沛 尹拓 杨娜 杨秀瑶 张梦洁 韩沛辰 奚登贤 刘小珍 张汉尧 DU Chao-jin;LI Yi-pei;YIN Tuo;YANG Na;YANG Xiu-yao;ZHANG Meng-jie;HAN Pei-chen;XI Deng-xian;LIU Xiao-zhen;ZHANG Han-yao(Key Laboratory of Forest Genetic Improvement and Breeding/The Key Laboratory of Biodiversity Conservation of Southwest China,National Forestry and Grassland Administration,College of Forestry,Southwest Forestry University,Kunming 650224)

机构地区：[1]西南林业大学林学院西南地区生物多样性保育国家林业与草原局重点实验室/云南省高校林木遗传改良与繁育重点实验室,昆明650224

出　　处：《植物遗传资源学报》2023年第1期296-306,共11页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(32160556)。

摘　　要：Hort16A黄肉猕猴桃是中华猕猴桃(Actinidia chinensis Planch.)的一个品种,其营养价值高,受到了广大消费者们的青睐,但该品种抗冻性不强,抗溃疡病、枝腐病能力差等原因严重影响了其推广种植。此外黄肉猕猴桃低温胁迫响应机制目前尚不清楚。本研究通过对低温胁迫的黄肉猕猴桃进行转录组分析,为了解其相关机制以及后续进行抗寒研究提供理论参考。以前期筛选出的抗寒四倍体植株作为材料,将低温(0℃)处理5 h的作为试验组和常温处理的作为对照组,取样利用Illumina NovaSeq 6000平台进行转录组测序。比较试验组和对照组的转录组数据共发现1630个基因表达差异达到显著水平,其中619个上调表达,1011个下调表达。GO富集分析表明1013个差异表达基因被富集到分子功能、细胞组分和生物过程三大功能中;KEGG富集分析表明410个差异基因被富集到植物激素信号转导、MAPK植物信号通路、淀粉和蔗糖代谢等89个代谢通路中。初步推定PYL、PSR、TPS、GH3、SAUR、PP2C等9个基因与黄肉猕猴桃抗寒响应有关。本研究结果可为进一步研究四倍体黄肉猕猴桃低温胁迫响应机制提供理论参考。Hort16A, a variety of Actinidia chinensis Planch., is highly popular among consumers due to its high nutritional value. However, this variety is sensitive to frost and susceptible to ulcer disease and branch rot.The response mechanism of yellow-fleshed A. chinensis upon cold temperature remains still unclear. In this study, transcriptome analysis of yellow-fleshed kiwifruit under low temperature stress was performed to provide theoretical references for understanding the mechanisms involved and subsequent studies on cold resistance. The cold-resistant tetraploid materials and the plants treated at low temperature(0 ℃) for 5 h were used as the experimental group, and those not specially treated at room temperature were used as the control group, and the samples were taken for transcriptome sequencing using Illumina NovaSeq 6000 platform. GO enrichment analysis revealed 1013 differentially expressed genes, which were enriched into three categories: molecular function, cellular component, and biological process. KEGG enrichment analysis showed that 410 differentially expressed genes were enriched in the three main functions of plant hormone signal transduction, MAPK signaling pathway-plant, starch and sucrose metabolism, and 89 other metabolic pathways. Nine genes related to cold resistance in yellow-fleshed kiwifruit, including PYL, PSR, TPS, GH3, SAUR, and PP2C, were tentatively identified. The results of this study can provide theoretical references for further studies on the response mechanism of tetraploid yellow-fleshed kiwifruit to low temperature stress.

关 键 词：Hort16A黄肉猕猴桃 四倍体 转录组 抗寒基因 

分 类 号：S663.4[农业科学—果树学]