一株拮抗链霉菌SM3-7的鉴定及其对玉米茎基腐病的防效  

Identification and Control Effect of Antagonistic Streptomyces SM 3-7 against Maize Stalk Rot Disease

作　　者：侯翔宇 吴瀚翔 张杰 黄鹏[1] 姚锦爱[1,2] 余德亿 HOU Xiangyu;WU Hanxiang;ZHANG Jie;HUANG Peng;YAO Jinai;YU Deyi(Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Fujian Engineering Research Center for Green Pest Management/Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;State Key Laboratory for Biology of Plant Disease and Insect Pests/Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094,China)

机构地区：[1]福建省作物有害生物监测与治理重点实验室/福建省农业科学院植物保护研究所/福建省作物有害生物绿色防控工程研究中心,福州350013 [2]植物病虫害生物学国家重点实验室/中国农业科学院植物保护研究所,北京100094

出　　处：《中国生物防治学报》2022年第6期1526-1533,共8页Chinese Journal of Biological Control

基　　金：植物病虫害生物学国家重点实验室项目(SKLOF202125);福建省自然科学基金项目(2020J011357);福建省农业科学院科技创新团队建设项目(STTT2021-2-2);福建省人民政府中国农业科学院农业高质量发展超越“5511”协同创新工程(XTCXGC2021011,XTCXGC2021017)。

摘　　要：为获得对玉米茎基腐病具有良好防效的拮抗菌株,从玉米根际土壤中分离以玉米茎基腐病拟轮枝镰孢菌Fusarium verticillioides为靶标菌的拮抗放线菌菌株。采用平板对峙法测定不同菌株的抑菌活性,用抑菌圈法筛选效果良好的拮抗菌株并进行种类鉴定、抑菌谱和防效测定。结果表明,从土壤中分离并筛选获得1株对拟轮枝镰孢菌具有明显抑制作用的菌株SM3-7。经形态学、生理生化特征和16S rDNA系统发育分析,将菌株SM3-7鉴定为核粒链霉菌Streptomyces sclerogranulatus。该菌株抑菌谱广,对玉米上的8种植物病原菌有良好的抑制作用,对拟轮枝镰孢菌抑菌圈直径可达(28.03±0.45)mm。盆栽防效结果表明,菌株SM3-7发酵液对抗病品种“良玉99”和感病品种“全玉1233”的玉米茎基腐病14 d防效分别达77.28%和76.12%,21 d防效达72.20%和71.89%。核粒链霉菌菌株SM3-7发酵液可有效防治玉米茎基腐病,为利用微生物防治玉米茎基腐病拟轮枝镰孢菌提供参考。In order to obtain an antagonistic bacterium with good control effect against maize stalk rot disease,the antagonistic bacteria were isolated from the rhizosphere soil of maize,with Fusarium verticillioides as the target fungi.The antibacterial activity of different strains was determined by the confrontation method,and the strains with the best antibacterial effect were selected by inhibition zone method.The selected strains were identified and its bacteriostatic spectrum and control effect were determined.The results showed that the strain SM3-7 had obvious inhibitory effect on F.verticillioides.Based on morphological,physiological,biochemical characteristics and 16S rDNA phylogenetic analysis,the strain SM3-7 was identified as Streptomyces sclerogranulatus.This strain had a broad antibacterial spectrum and had good inhibitory effect against 8 plant pathogens on corn,and inhibition zone diameter was(28.03±0.45) mm against F.verticillioides.In pot experiment,the control efficacies of strain SM3-7 against maize stalk rot on ‘Liangyu 99’ and ‘Quanyu 1233’ varieties reached 77.28% and 76.12%in 14 days,77.28% and 76.12% in 21 days,respectively.The results showed that SM3-7 fermentation broth could effectively control maize stalk rot,which could lay a theoretical foundation for the development of biological control products against maize stalk rot.

关 键 词：玉米茎基腐病 核粒链霉菌 筛选 鉴定 生物防治 

分 类 号：S476[农业科学—农业昆虫与害虫防治]