几种常用化学杀虫剂对Bt菌株生长影响的初步研究  

A Preliminary Study on the Effects of Several Frequently Used Chemical Insecticides on the Growth of Diverse Bacillus thuringiensis Strains

作　　者：束长龙[1] 张贤 王奎[1] 曹蓓蓓[1] 张杰[1] SHU Changlong;ZHANG Xian;WANG Kui;CAO Beibei;ZHANG Jie(State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室

出　　处：《中国生物防治学报》2022年第5期1166-1173,共8页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2017YFD0201204);国家自然科学基金(31872935)。

摘　　要：苏云金芽胞杆菌(Bacillusthuringiensis,Bt)可产生多种杀虫活性物质,是一种应用最广泛的微生物杀虫剂。最近研究表明Bt对化学杀虫剂抗性害虫有较好的防治效果,并且与化学杀虫剂的联合使用可以提高杀虫效果,减少化学杀虫剂的用量。为了进一步设计更好的Bt与化学杀虫剂协同控害方案,本论文研究了不同亚种的Bt菌株与常用化学杀虫剂的兼容性。泛基因组分析表明不同亚种的Bt菌株在基因组成上有较大差异,说明Bt菌株对化学杀虫剂的反应可能会有所不同。进一步化学杀虫剂对Bt增殖影响分析结果显示,溴虫腈对大部分Bt测试菌株有抑制,说明Bt与化学杀虫剂协同使用时需要测定两者兼容性。高效氯氰菊酯对所有常用的防治鳞翅目害虫的Bt杀虫剂亚种类型库斯塔克亚种(Bt kurstaki,Btk)和鲇泽亚种(Bt aizawai,Bta)菌株影响都不大,可以进行协同使用;氯虫苯甲酰胺和茚虫威对Btk菌株HD1有一定抑制作用,定虫隆对其有较好的促进作用,说明HD1与定虫隆协同使用效果更好;茚虫威对Bta菌株G03影响较小,定虫隆对其有一定的抑制作用,氯虫苯甲酰胺对G03则有一定的促进作用,说明G03及其衍生菌株可以与氯虫苯甲酰胺、茚虫威协同使用。本研究结果说明Bt菌株对不同杀虫剂的敏感性是有差异的,可以通过筛选获得兼容性好的组合,为进一步制定Bt杀虫剂与化学杀虫剂配伍方案与协同使用技术提供了理论指导与数据参考,对实现化学农药减量施用具有重要的指导意义。Bacillus thuringiensis(Bt) can produce a variety of pesticidal active substances and is one of the most widely used microbial insecticides.Recent studies have shown that Bt is of high control of chemical insecticide resistant pests and that combined use of Bt with chemical insecticides can improve the control efficiency and reduce the usage of chemical insecticides.To improve the synergistic pest control between Bt and chemical insecticides,this study investigated the compatibility of different subspecies of Bt strains with frequenctly used chemical insecticides.Pan-genomic analysis showed that Bt strains from different subspecies differed significantly in their genetic composition,suggesting that Bt strains may respond differently to chemical insecticides.Further analysis of the effect of chemical insecticides on Bt proliferation showed that chlorfenapyr inhibited most of the tested Bt strains,indicating that the compatibility between Bt and chemical insecticides needs to be measured when they are used in combination.The Bt insecticide subspecies Bt kurstaki(Btk) and Bt aizawai(Bta) frequently used for the control of lepidopteran pests showed synergistical control with beta-cypermethrin.To Btk strain HD1,chlorantraniliprole and indoxacarb exhibited some inhibitory effect,while chlorfluazuron promoted growth,indicating that HD1 and chlorfluazuron can be used synergistically.For Bta strain G03,indoxacarb had less negative effect,chlorfluazuron was inhibitory,and chlorantraniliprole was promotional,showing that G03 and its derivatives can be used synergistically with chlorantraniliprole and indoxacarb.The results indicate that the sensitivity of Bt to chemical insecticides differ between strains,indicating that combinations between Bt and chemicals with high compatibility can be obtained through screening.Our data also shed light on the further combination of Bt insecticide and chemical insecticide,which is important for the reduction of chemical pesticide application.

关 键 词：苏云金芽胞杆菌 化学杀虫剂 增殖影响 农药配伍 害虫防治 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]