烟草浸提液对番茄晚疫病菌的化感抑制效应机理研究  

Mechanism of Allelopathic Inhibition Effect of Tobacco(Nicotiana tabacum L.)Extracts on Phytophthora infestans

作　　者：刘嘉方 李蕾 云兴福[1] LIU Jiafang;LI Lei;YUN Xingfu(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010010,China;Baotou Teachers'College,Inner Mongolia University of Science&Technology,Baotou 014030,China;Manzhouli Russian Vocational College,Manzhouli 021400,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,呼和浩特010019 [2]内蒙古科技大学包头师范学院,包头014030 [3]满洲里俄语职业学院,满洲里021400

出　　处：《中国生物防治学报》2022年第5期1231-1241,共11页Chinese Journal of Biological Control

基　　金：内蒙古自治区科技成果转化项目(CGZH2018140);内蒙古自治区应用技术研究与开发重点项目(201702068)。

摘　　要：为探究烟草对番茄晚疫病菌的抑制效果及作用机制,采用烟草根、叶浸提液对番茄晚疫病菌每代处理并连续培养多代,每一代测定菌落直径和致病力,以筛选获得弱毒菌株;在培养基条件下研究弱毒菌株的生理变化和菌体形态并进行转录组测序。结果表明,在连续8代烟草浸提液作用下,各处理病菌的菌落直径减小且致病力降低。其中PI-R处理第7、8代的病情指数<10,据此确定第8代PI-R处理为番茄晚疫病菌弱毒菌株PIAS-R。与CK菌株相比,PIAS-R菌株的孢子萌发率、生物量和细胞壁降解酶活性显著降低,菌丝体形态明显畸变。转录组分析表明,在PIAS-R菌株中分别有1086/1565个基因显著上调/下调;差异表达基因在44个GO功能条目和83条KEGG通路中得到富集。烟草通过抑制番茄晚疫病菌生理性状,破坏菌体形态结构,影响内部基因表达,导致其对寄主的致病力减弱。In order to explore the inhibitory effect and mechanism of tobacco on Phytophthora infestans,the extracts of tobacco roots and leaves were used to treat P.infestans in each generation and cultured continuously for multiple generations,and the colony diameter and virulence of each generation were measured to screen the attenuated strain.The physiological changes and mycelium morphology of the attenuated strain were investigated under medium conditions,and the transcriptome was sequenced.The results showed that the colony diameter and virulence of P.infestans in each treatment were reduced under consecutive treatment by tobacco extracts for 8generations.Among them,the disease index of the 7th and 8th generations of PI-R treatment was less than 10,so it was determined that the 8th generation of PI-R treatment was an attenuated strain PIAS-R of P.infestans.Compared with CK strain,the spore germination rate,biomass,and cell wall degrading enzymes activities of PIAS-R strain decreased significantly,and the mycelium morphology was obviously distorted.Transcriptomic analysis showed that 1086/1565 genes were significantly up/down-regulated in PIAS-R strain,respectively.Differentially expressed genes were enriched in 44 GO functional terms and 83 KEGG pathways.Tobacco could inhibit the physiological characteristics,destroy the mycelium morphological structure,and affect internal genes expression of P.infestans,which leads to the weakening of its virulence to the host.

关 键 词：烟草 番茄晚疫病菌 致病力 菌体生理和形态特征 转录组测序 

分 类 号：S476[农业科学—农业昆虫与害虫防治]