浙麦冬黑斑病病原菌的分离鉴定及淀粉酶产色链霉菌对其生物防治效果  

Isolation and Identification of Pathogen of Ophiopogon japonicus Black Spot and Control Effect of Streptomyces diastatochramogenes

作　　者：徐亦雯 张诺 曹瑱艳 申屠旭萍 俞晓平 XU Yiwen;Zhang Nuo;CAO Zhenyan;SHENTU Xuping;Yu Xiaoping(Zhejiang Provincial Key Laboratory of Biometrology and Inspection&Quarantine/College of Life Science,China Jiliang University,Hangzhou 310018,China)

机构地区：[1]中国计量大学生命科学学院/浙江省生物计量及检验检疫技术重点实验室,杭州310018

出　　处：《中国生物防治学报》2022年第5期1280-1287,共8页Chinese Journal of Biological Control

基　　金：浙江省公益技术研究项目(2017C32006,2018C02030);中国计量大学科研业务费项目学生科技创新专项(2021YW95,2022YW104)。

摘　　要：黑斑病是浙江省慈溪市浙麦冬种植基地常发病害,传染速度快且严重影响麦冬的产量与品质。为了明确引起黑斑病的病原菌以便后续有效防治该病害,本文通过对染病组织培养、病原菌分离纯化和柯赫法则验证、形态学观察与基因序列分析比对(ITS、EF-1α、RPB2、Alt a 1、His 3、ATP),最终鉴定出引起浙麦冬黑斑病的病原菌是链格孢菌Alternariaalternata。研究淀粉酶产色链霉菌Streptomyces diastatochromogenes 1628代谢产物对黑斑病的防效结果表明,1628代谢产物对链格孢菌菌丝生长具有较强的抑制作用,其EC50=0.764%(体积浓度)。田间防效结果表明7和14 d校正防效分别可达74.08%和65.28%,均高于阳性对照多菌灵。本研究明确了浙麦冬黑斑病病原菌的分类地位,并筛选到能有效抑制该病原菌的生防菌株淀粉酶产色链霉菌,为后续浙麦冬黑斑病的防治提供依据。Black spot disease is a common disease in the planting base of Ophiopogon japonicus in Cixi City,Zhejiang Province.It spreads rapidly and seriously affects the yield and quality of O.japonicus.In order to effectively control the disease,the infected tissue culture,pathogen isolation and purification,Koch’s rule verification,the pathogen morphological observation and gene sequence analysis(ITS,TEF-1α,RPB2,Alt a 1,His 3,ATP) were carried out,and the pathogen of O.japonicus black spot was identified as Alternaria alternata.The metabolites of Streptomyces diastochromogenes 1628 obtained in previous study were used for O.japonicus black spot biocontrol.The results showed that S.diastochromogenes 1628 had a strong inhibitory effect on mycelial growth of A.alternata,with EC50 value of 0.764%(the ratio of volume).The corrected control efficacies on black spot of O.japonicus 7 and 14 days after treatment were 74.08% and 65.28%,respectively.The above results provide a basis for the subsequent prevention and control of O.japonicus black spot.

关 键 词：浙麦冬 黑斑病 链格孢菌 病原菌鉴定 淀粉酶产色链霉菌1628 

分 类 号：S476[农业科学—农业昆虫与害虫防治]