Nitrogen starvation induces genome-wide activation of transposable elements in Arabidopsis  

作　　者：Yue Wang Yi Liu Shaofeng Qu Wenjie Liang Linhua Sun Dong Ci Zhitong Ren Liu-Min Fan Weiqiang Qian 

机构地区：[1]State Key Laboratory of Protein and Plant Gene Research,School of Advanced Agricultural Sciences,Peking University,Beijing 100871,China [2]School of Life Sciences,Peking University,Beijing 100871,China [3]Peking University Institute of Advanced Agricultural Sciences,Weifang 261000,China

出　　处：《Journal of Integrative Plant Biology》2022年第12期2374-2384,共11页植物学报（英文版）

基　　金：the National Natural Science Foundation of China(31970614);the National Key R&D Program of China(2018YFE0204700)。

摘　　要：Nitrogen(N)availability is a major limiting factor for plant growth and agricultural productivity.Although the gene regulation network in response to N starvation has been extensively studied,it remains unknown whether N starvation has an impact on the activity of transposable elements(TEs).Here,we report that TEs can be transcriptionally activated in Arabidopsis under N starvation conditions.Through genetic screening of idm1-14 suppressors,we cloned GLU1,which encodes a glutamate synthase that catalyzes the synthesis of glutamate in the primary N assimilation pathway.We found that glutamate synthase 1(GLU1)and its functional homologs GLU2 and glutamate transport 1(GLT1)are redundantly required for TE silencing,suggesting that N metabolism can regulate TE activity.Transcriptome and methylome analyses revealed that N starvation results in genome-wide TE activation without inducing obvious alteration of DNA methylation.Genetic analysis indicated that N starvationinduced TE activation is also independent of other well-established epigenetic mechanisms,including histone methylation and heterochromatin decondensation.Our results provide new insights into the regulation of TE activity under stressful environments in planta.

关 键 词：DNA methylation glutamate synthase nitrogen starvation transcriptional gene silencing transposable element 

分 类 号：Q943[生物学—植物学]