Energy Pumping by Surface Collectors on Upconversion Nanoparticles for Extended Transfer and Efficient Self-Evaluable Photodynamic Therapy  

作　　者：Xiaobo Zhang Weiwei Chen Xiaoyu Xie Yue Zhang Zhicong Chao Haibo Ma Ying Liu Huangxian Ju 

机构地区：[1]State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023 [2]Institute of Theoretical and Computational Chemistry,Nanjing University,Nanjing 210023 [3]School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023 [4]Chemistry and Biomedicine Innovation Center,Nanjing University,Nanjing 210023

出　　处：《CCS Chemistry》2022年第4期1251-1262,共12页中国化学会会刊（英文）

基　　金：the National Natural Science Foundation of China(nos.21635005,21974064,22022405,21827812,21890741,22004062,and 22004068);Natural Science Foundation of Jiangsu Province for distinguished Young Scholars(no.BK20200010);Specially appointed Professor Foundation of Jiangsu Province;China Postdoctoral Science Foundation(no.252986);Program for Innovative Talents and Entrepreneurs of Jiangsu Province.

摘　　要：Although upconversion nanoparticle(UCNP)-based luminescence resonance energy transfer(LRET)has attracted intensive attention,its application in sensing strategies for biomacromolecule detection is still limited due to the low energy-transfer efficiency.Here,we develop an energy pumping strategy to enhance the upconversion LRET efficiency of UCNP from 3.6% to 70.3%.Surface collector dye cyanine-3(Cy3)is modified on the UCNP surface to concentrate the scattered emission energy from interior UCNP to particle surface and then further pumped to the surface extended acceptor.Using caspase-3 as a target,its recognition and cleavage of QSY7-labeled peptide co-assembled on UCNP-Cy3 leads to the release of QSY7 to recover Cy3 fluorescence.Owing to the multiple emissions of UCNP,this system can be integrated with another surface collector to enhance reactive oxygen species(ROS)generation for photodynamic therapy(PDT).Caspase-3,as a PDT product,is imaged in situ with an internal standard to self-evaluate the PDT effect.

关 键 词：upconversion nanoparticle luminescence resonance energy transfer energy collector photodynamic therapy therapeutic effect self-evaluation 

分 类 号：TB383[一般工业技术—材料科学与工程]