Unveiling Cellular Internalization Dynamics of Single Gold Nanorods by Tracking Their Orientational and Translational Motions  

作　　者：Dong Xu Yi Du Feng Ge Yan He Edward S.Yeung 

机构地区：[1]National Engineering Laboratory for Rice and By-Products Further Processing,College of Food Science and Engineering,Central South University of Forestry&Technology,Changsha 410004 [2]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,College of Biology,Hunan University,Changsha 410082 [3]Department of Chemistry,Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology(Ministry of Education),Tsinghua University,Beijing 100084 [4]Department of Chemistry,Iowa State University,Ames,IA 50011

出　　处：《CCS Chemistry》2022年第4期1295-1304,共10页中国化学会会刊（英文）

基　　金：supported by the National Natural Science Foundation of China(grant nos.21127009,21221003,and 21425519);the Training Program for Excellent Young Innovators of Changsha(grant no.kq1905061);the Natural Science Foundation of Hunan Province,China(grant no.020RC3042).

摘　　要：Single nanoparticle tracking(SPT)is a unique and powerful tool to investigate the interaction between nanoparticles and cells,which is of considerable importance for nanotechnology applications in biomedical fields and in-depth understanding of biological activities.However,previous work typically focused on translations of single nanoparticles while they undergo both translational and rotational motions.In this study,we obtained both the translational and rotational dynamics of single gold nanorods during their cellular internalization process using dual-channel polarization microscopy.In particular,the azimuth and polar angles were integrated into a polar coordinate systemto obtain three general orientation distribution patterns,found to have a close relationship with the nanoparticle cellular internalization process and time-dependent alterations.Moreover,the patterns accompanied by trajectories,translational and rotational coefficients,the azimuth and polar angles,and other parameters provided a wealth of knowledge on the nanoparticle cellular internalization dynamics with unprecedented details.We observed that the gold nanorods could initially assume a tip-first quick rotation state with partially restricted orientations,then change to a strongly confined near-vertical insertion state with slight angular fluctuations,and eventually transform into a random and fast rotation state.Our methodology opens up a new avenue for a detailed understanding of biological processes.

关 键 词：orientation distribution pattern nanoparticle cellular internalization orientational and translational motion single nanoparticle tracking 

分 类 号：TB383[一般工业技术—材料科学与工程]