An Aggregation-Induced Emission Optical Highlighter for the Studies of Endoplasmic Reticulum-Lipid Droplet Content Dynamics  

作　　者：Engui Zhao Chuen Kam Ming-Yu Wu Erjing Wang Jiawei Wang Ben Zhong Tang Sijie Chen 

机构地区：[1]School of Science,School of Electronics and Information Engineering,Harbin Institute of Technology,Shenzhen,HIT Campus of University Town,Shenzhen 518055 [2]Ming Wai Lau Centre for Reparative Medicine,Karolinska Institutet,Hong Kong 999077 [3]Overseas Expertise Introduction Center for Discipline Innovation(D18025),Collaborative Innovation Center for Advanced Organic Chemical Materials Co-Constructed by the Province and Ministry,School of Material Science and Engineering,Hubei University,Wuhan 430062 [4]Department of Chemistry,The Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction,SCUT-HKUST Joint Research Institute,Institute for Advanced Study,Department of Chemical and Biological Engineering,The Hong Kong University of Science and Technology,Clear Water Bay,Kowloon,Hong Kong 999077 [5]Shenzhen Institute of Molecular Aggregate Science and Engineering,School of Science and Engineering,Guangdong of Electronic and Information Engineering,The Chinese University of Hong Kong,Shenzhen,2001 Longxiang Boulevard,Longgang District,Shenzhen City,Guangdong 518172

出　　处：《CCS Chemistry》2022年第2期515-525,共11页中国化学会会刊（英文）

基　　金：the National Natural Science Foundation of China(no.22005050)for its financial support;E.W.acknowledges the financial support from National Natural Science Foundation of China(no.52073082);Hubei Provincial Natural Science Foundation of China(no.2017CFB489);J.W.acknowledges financial support from GuangDong Basic and Applied Basic Research Foundation Regional Joint Fund(grant no.2020A1515110328);B.Z.T and S.C.acknowledge the Collaborative Research Fund(CRF)of the University Grants Committee(C6014-20W)for their financial support.

摘　　要：The lipid droplet(LD)is a dynamic organelle responsible for lipid storage and metabolism that plays important roles in maintaining lipid homeostasis.However,limited strategies are available for tracking the LD content exchange.In this contribution,we report a novel fluorescent probe,TPEAmAl,for real-time LD content dynamics tracking.TPE-AmAl is LD-specific,but emits faintly due to its intramolecular motion.Upon photoactivation,it undergoes a photocyclodehydrogenation reaction and shows a large fluorescence increment.Thus,it can be used for highlighting selected LDs with high spatial resolution.By measuring the fluorescence changes in the distal region,the lipid content exchange efficiency can be estimated.In our experiment,LD content exchange rate differences between nascent and mature LDs as well as cells with normal and deficient LD budding machinery are observed.This probe expands the fluorescence-based toolbox for LD content dynamics studies.

关 键 词：aggregation-induced emission lipid droplet-ER content dynamics optical highlighter PHOTOACTIVATION 

分 类 号：O62[理学—有机化学]