温度对低盐驯化褶皱臂尾轮虫生活史参数的影响  

Effects of temperature on life history parameters of the rotifer Brachionus plicatilis domesticated under low salinity

作　　者：崔宇翔 张树林 刘毅 罗颖[3] 祁洪芳[3] 杨建新[3] 吴洪芳[3] 张清靖 CUI Yuxiang;ZHANG Shulin;LIU Yi;LUO Ying;QI Hongfang;YANG Jianxin;WU Hongfang;ZHANG Qingjing(Key Laboratory of Aquaecology and Aquaculture of Tianjin,College ofFisheries,Tianjin Agricultural University,Tianjin 300384,China;Beijing Key Laboratory of Fishery Biotechnology,Fisheries ScienceInstitute,Beijing Academy of Agriculture and Forestry Sciences,Beijing100068,China;Qinghai Key Laboratory of Qinghai-Lake Naked CarpsBreeding and Conservation,Rescue and Rehabilitation Center of Naked Carpsof Qinghai Lake,Xining 810016,China)

机构地区：[1]天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300384 [2]北京市农林科学院水产科学研究所,渔业生物技术北京市重点实验室,北京100068 [3]青海湖裸鲤救护中心,青海省青海湖裸鲤繁育与保护重点实验室,青海西宁810016

出　　处：《大连海洋大学学报》2022年第6期968-976,共9页Journal of Dalian Ocean University

基　　金：国家重点研发计划“蓝色粮仓科技创新”项目(2020YFD0900103);现代农业产业技术体系北京市创新团队项目(BAIC07-2022-06);北京市农林科学院青年基金(QNJJ202020)。

摘　　要：为探索低盐驯化褶皱臂尾轮虫Brachionus plicatilis规模化扩繁的最适温度范围,以盐度23条件下培养的褶皱臂尾轮虫和低盐(盐度6)驯化后的褶皱臂尾轮虫为试验对象,设置6个温度(18、20、22、24、26、28℃)组,采用单个体培养法研究了不同温度对两个盐度组褶皱臂尾轮虫生活史参数的影响。结果表明:随温度升高,两个盐度组轮虫的生命期望(e_(0))、世代时间(T)、净生殖率(R_(0))、内禀增长率(r_(m))、平均寿命和产卵量整体上呈现增加趋势;盐度6组轮虫的生命期望、平均寿命和世代时间均在26℃下最大,而净生殖率、内禀增长率和产卵量则在28℃时最大;在相同温度下,盐度6组轮虫的生命期望、平均寿命均与盐度23组轮虫无显著性差异(P>0.05);除20℃外,其余温度下,盐度6组轮虫的净生殖率显著低于盐度23组(P<0.05),但两个盐度组间世代时间无显著性差异(P>0.05);两个盐度组轮虫的种群内禀增长率在18、20℃下无显著性差异(P>0.05),在其余温度下有显著性差异(P<0.05);除20、22℃外,其余温度下,盐度23组轮虫的产卵量均显著高于盐度6组(P<0.05)。研究表明,在温度为26~28℃时,低盐驯化的褶皱臂尾轮虫在低盐培养液中具有规模化生产的可行性。In order to explore the optimal temperature range of rotifer Brachionus plicatilis domesticated under low salinity for large-scale cultivation, the life history parameters were determined in the rotifer domesticated at a salinity of 6(test group)and 23(control group)for 1 year at temperature of 18, 20, 22, 24, 26, and 28 ℃ to evaluate the effects of temperature on the life history parameters of the rotifer by individual culture method. The results showed that the rotifer in the two salinity groups had increase in life expectancy(e_(0)), generation time(T), net reproductive rate(R_(0)), intrinsic growth rate(r_(m)), average life span and egg production with the increase in temperature, with the maximal values of e0, average life span and T at 26 ℃, and the maximal R0, rmand egg production at 28 ℃ in the rotifer domesticated under low salinity at salinity of 6. There were not significantly different e0and average life span in the rotifer at salinity of 6 from rotifer at salinity of 23 under the same temperature(P>0.05). However, under the same temperature except 20 ℃, the R0of rotifer at salinity of 6 was found to be significantly lower than that in the rotifer at salinity of 23(P<0.05), without significant difference in T between the two salinity groups(P>0.05). There was no significant difference in the rmof the rotifer in the two salinity groups at 18 ℃ and 20 ℃(P>0.05), but there were significant differences in the other temperature groups(P<0.05). There was no significant difference in egg production in the rotifer in the two salinity groups at 20 ℃ and 22 ℃(P>0.05), in the other temperature groups, however, significantly higher egg production in the rotifer at salinity of 23 than that at salinity of 6(P<0.05). In conclusion, in the temperature ranging from 26 ℃ to 28 ℃, large-scale production of rotifer B.plicatilis domesticated to salinity 6 is feasible. The findings provide basic data for the large-scale cultivation of rotifer B.plicatilis in low-salt water by low salinity acclimation.

关 键 词：褶皱臂尾轮虫 低盐驯化 温度 盐度 生活史特征 

分 类 号：S963[农业科学—水产养殖]