Investigation of photodamage by femtosecond laser to cells via gold nanorods  

作　　者：Yang Li Hao He Huanyu Song Bowen Liu Mingle Hu Chingyue Wang 

机构地区：[1]Ultrafast Laser Laboratory Key Laboratory of Optoelectronic Information Technology(Ministry of Education)College of Precision Instrument and Optoelectronics Engineering Tianjin University,Tianjin 300072,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2017年第1期68-74,共7页创新光学健康科学杂志（英文）

基　　金：from National Natural Science Foundation of China(NSFC)81571719,61322502;Open Project of State Key Laboratory of Modern Optical Instrumentation,Zhejiang University,and Program for Changjiang Scholars and Innovative Research Team in Uni-versity IRT13033.

摘　　要：Usually,only focused femtosecond(fs)lasers at near-infrared(NIR)range can induce photo-damage to transparent cells,making it difficult to treat large amount of cells by such optical methods for photostimulation.In this study,we clarify the mechanism of photodamage to cells that are co-cultured with gold nanorods(GNRs)by fs laser.The pulse duration and repetition rate of the fs laser play a key role in cell damage suggesting that the heat accumulation con-tributes to the major part for the cell damage rather than the high peak power which mainly determines the efficiency of multiphoton excitation.We further show that cellular Ca^(2+)can also be released in this scheme,but the process is more sensitive to peak power.Our results can provide a large-scale GNR-mediated photostimulation for cell signaling modulation.

关 键 词：NANORODS photothermal effect thermo-optical materials ultrafast lasers. 

分 类 号：TN2[电子电信—物理电子学]