In vivo fiber photometry of neural activity in response to optogenetically manipulated inputs in freely moving mice  

作　　者：Liang Li Yajie Tang Leqiang Sun Khaista Rahman Kai Huang Weize Xu Jinsong Yu Jinxia Dai Gang Cao 

机构地区：[1]State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan 430070,P.R.China [2]College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070,P.R.China [3]Bio-Medical center Huazhong Agricultural University Wuhan 430070,P.R.China [4]Key Laboratory of Development of Veterinary Diagnostic Products Ministry of Agriculture,College of Veterinary Medicine Huazhong Agricultural University,Wuhan 430070,P.R.China [5]Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan,430074,P.R.China [6]Convergence Technology Co.Ltd 1037 Luoyu Road,Hongshan District,Wuhan 430074,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2017年第5期47-58,共12页创新光学健康科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos.31371106 and 91632110);HZAU Independent Innovation Fund (2014BQ019).

摘　　要：In vito fber photometry is a powerful technique to analyze the dy namics of population neurons during fiunctional study of neuroscience.Here,we introduced a detailed protocol for fiber photometry-based calciun reording in freely moving mice,covering from virus injection,fiber stub insertion,optogenetical stimulation to data procurement and analysis.Furthemnore,we applied this protocol to explore neuronal activity of mice latenal-posterior(LP)thalaric nucleus in response to optogenetical stimulation of primary visual cortex(V1)neurons,and explore axon clusters activity of optogenetically evoked V1 neurons.Final confirmation of virus-based protein expression in V1 and precise fber insertion indicated that the surgery procedure of this protocol is reliable for functional calcium recording.The scripts for data analysis and some tips in our protocol are provided in details.Together,this protocol is simple,low-cost,and effective for neuronal activity detection by fiber photometry,which will hep neuroscience researchers to carry out fiunctional and behavioral study in vivo.

关 键 词：Fiber photometry surgical operation optogenetical stimulation neural activity freely moving recording 

分 类 号：TN9[电子电信—信息与通信工程]