1株仔猪腹泻产超广谱β-内酰胺酶大肠杆菌检测  

Detection of extended-spectrum β-lactamase producing Escherichia coli causing piglet diarrhea

作　　者：韩彦飞 邵冰[2] 富杨 宋淼[2] 郭晓亮[2] 吕明哲 夏士亮[2] 赵福庆 Han Yanfei;Shao Bing;Fu Yang;Song Miao;Guo Xiaoliang;LüMingzhe;Xia Shiliang;Zhao Fuqing(Liaoning Agricultural Technical College,Liaoning Yingkou 115009;Northeast Agricultural University,Heilongjiang Harbin 150030;Heze Vocational College,Shandong Heze 274000;Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences,Heilongjiang Qiqihar 161005)

机构地区：[1]辽宁农业职业技术学院,辽宁营口115009 [2]东北农业大学,黑龙江哈尔滨150030 [3]菏泽职业学院,山东菏泽274000 [4]黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔161005

出　　处：《现代畜牧兽医》2022年第11期11-14,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：研究旨在为发生仔猪腹泻的某养猪场科学合理用药提供参考。试验从腹泻仔猪粪便及死亡仔猪器官样本中分离出大肠杆菌,对该分离株进行药敏试验、致病性试验及耐药基因检测。结果显示,分离株为携带K99黏附素的产肠毒素大肠杆菌,对头孢噻肟、头孢曲松及头孢克肟等β-内酰胺类药物耐药严重。通过耐药基因检测发现,分离株大肠杆菌携带blaCTX-M耐药基因,为产超广谱β-内酰胺酶大肠杆菌,可产生超广谱β-内酰胺酶水解β-内酰胺类药物,从而产生耐药性。The purpose of the study was to provide reference for scientific and rational drug use in a pig farm with piglet diarrhea. Escherichia coli was isolated from diarrhea piglets’ feces and dead piglets’ organ samples. The drug sensitivity test,pathogenicity test and drug resistance gene detection were carried out on the isolated strain. The results showed that the isolates were enterotoxigenic Escherichia coli carrying K99 adhesin, which were highly resistant to cefotaxime, ceftriaxone and cefixime β-lactam drugs. Through drug resistance gene detection, it was found that the isolated Escherichia coli carried blaCTX-M drug resistance gene, which was an ultra-broad spectrum β-lactamase producing ultra-broad spectrum β-lactamase hydrolyzing β-lactamases, resulting in drug resistance.

关 键 词：Β-内酰胺类 大肠杆菌 耐药基因 仔猪腹泻 

分 类 号：S852.6[农业科学—基础兽医学]