基于ALS-SOD1^(G93A)小鼠的腓肠肌病理分析探讨健脾通络方对ALS的干预作用  

Intervention Effect of Jianpi Tongluo Decoction on ALS Based on Pathological Analysis of Gastrocnemius Muscle of ALS-SOD1^(G93A) Mice

作　　者：滕展 刘树民[1] 张娜[1] TENG Zhan;LIU Shumin;ZHANG Na(Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2023年第2期21-26,共6页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金青年基金项目(8200424);黑龙江省自然科学基金(LH2020H081);黑龙江省中医药学会青年科技创新基金(ZHY19-013);黑龙江中医药大学博士创新基金(051761);黑龙江中医药大学研究生创新基金(2022yjscx009)。

摘　　要：目的:探讨健脾通络方对ALS-SOD1^(G93A)小鼠3个病程时期坐骨神经退化及腓肠肌萎缩改变的干预作用。方法:选取雄性ALS-SOD1^(G93A)转基因小鼠,按照疾病发展分为3个病程时期,分别为发病早期(60 d),发病中期(100 d)和发病末期(140 d),每个疾病时期分为4组,空白组(K组)7只、模型组(M组)6只、给药组(G组)7只与阳性药组(Y组)7只,共27只,3个发病时间段共81只。观察ALS-SOD1^(G93A)小鼠3个时期节点的步长变化;取ALS-SOD1^(G93A)小鼠3个时期的腓肠肌肌肉进行称重,统计学计算分析腓肠肌湿重百分比变化并进行HE病理染色观察其病理改变;记录发病末期(140 d)各组小鼠的坐骨神经退行性病变。结果:在3个疾病时期中,空白组步长、腓肠肌湿重比、HE病理染色无明显变化;模型组随着疾病发展进程逐渐严重;给药组及阳性药组与模型组比较有所回调。FJC染色在ALS-SOD1^(G93A)小鼠发病末期(140 d)空白组无明显病理变化,模型组FJC阳性退行性神经元增多,给药组及阳性药组与模型组比较有所回调。结论:健脾通络方对ALS-SOD1^(G93A)小鼠3个病程时期腓肠肌萎缩程度及发病末期(140 d)坐骨神经退变有干预作用。Objective:To investigate the intervention effect of Jianpi Tongluo Formula on sciatic nerve degeneration and gastrocnemius muscle atrophy in ALS-SOD1^(G93A) mice at different three disease phases.Methods:Male ALS-SOD1^(G93A) mice were randomly divided into early onset(60 d),middle onset(100 d),and end onset(140 d)according to disease development,which were then divided into the blank group(n=7),the model group(n=6),the medication group(n=7)and the positive drug group(n=7).The step length of mice was observed at the three different phases.The gastrocnemius muscles of ALS-SOD1^(G93A) mice were weighed at the three phases,and the percentage of wet weight of the gastrocnemius muscle was statistically calculated and analyzed,and the pathological changes were observed by HE staining.The sciatic nerve degeneration of ALS-SOD1^(G93A) mice at the end of the disease(140 d)was recorded by FJC fluorescence staining.Results:There were no significant pathological changes in step length,wet weight ratio of gastrocnemius muscle and pathological staining of HE in the blank group during the three disease periods;of which the model group became more and more severe with the development of the disease,and the medication group and the positive drug group could revered the changes when compared with the model group.There was no significant pathological change in FJC in the blank group at the end of disease(140 d);the number of FJC-positive degenerative neurons increased in the model group,and there was a correction in the medication group and the positive drug group compared with the model group.Conclusion:Jianpi Tongluo Formula has an intervention effect on gastrocnemius muscle atrophy and sciatic nerve degeneration at the end of disease in ALS-SOD1^(G93A) mice.

关 键 词：健脾通络方 行为学步长 腓肠肌重量 坐骨神经退化 

分 类 号：R285.5[医药卫生—中药学]