反相高效液相色谱法测定加味四妙勇安汤中绿原酸和梓醇的含量  

Determination of Chlorogenic Acid and Catalpol in Modified Simiao Yong’an Decoction by Reversed Phase High-Performance Liquid Chromatography

作　　者：李慧红 李敏 关红亚 韩鹏黎 刘嘉 成龙 曹巍 周丽娟 LI Huihong;LI Min;GUAN Hongya;HAN Pengli;LIU Jia;CHENG Long;CAO Wei;ZHOU Lijuan(Translational Medicine Center,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Zhengzhou 450007,China;Dept.of Pharmacy,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Zhengzhou 450007,China;Analytical Chemistry Research Center,Institute of Medicinal Plant,Chinese Academy of Medical Sciences,Beijing 100094,China)

机构地区：[1]郑州大学附属郑州中心医院转化医学中心,郑州450007 [2]郑州大学附属郑州中心医院药学部,郑州450007 [3]中国医学科学院药用植物研究所分析研究中心,北京100094

出　　处：《中国医院用药评价与分析》2023年第1期36-39,44,共5页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：2022年河南省科学科技攻关计划项目(No.222102310224);河南省高等学校重点科研项目计划(No.21B360010)。

摘　　要：目的:建立反相高效液相色谱法测定加味四妙勇安汤浸膏中绿原酸和梓醇含量的方法。方法:色谱柱为Merck Purospher STARLP RP-18 endcapped柱(250 mm×4.6 mm,5μm),流速为1.0 mL/min,柱温为35℃。(1)绿原酸的色谱条件,流动相为乙腈-0.4%磷酸(V∶V=11∶89),检测波长为327 nm。(2)梓醇的色谱条件,流动相A为乙腈,流动相B为0.1%磷酸,梯度洗脱(0~15 min,0.8%A;15~60 min,2%A),检测波长为210 nm。结果:绿原酸在4.420~442.0μg/mL(r=0.9998)范围内质量浓度与峰面积成良好线性关系;平均回收率为99.38%(RSD=1.26%)。梓醇在9.760~488.0μg/mL(r=0.9999)范围内质量浓度与峰面积成良好线性关系,平均回收率为99.34%(RSD=1.43%)。绿原酸和梓醇的最低检测限分别为0.40和0.82 ng,定量限分别为1.20和2.44 ng,精密度RSD分别为0.71%(n=6)和0.45%(n=6)。结论:本方法操作简便,分离效果和重复性好,准确度高,可用于加味四妙勇安汤提取物的质量评价。OBJECTIVE:To establish the reversed phase high-performance liquid chromatography method for the determination of chlorogenic acid and catalpol in modified Simiao Yong’an decoction.METHODS:The chromatographic column was Merck Purospher STARLP RP-18 endcapped column(250 mm×4.6 mm,5μm),the flow rate was 1.0 mL/min,the column temperature was 35℃.(1)The chromatographic conditions of chlorogenic acid were acetonitrile-0.4%phosphoric acid(V∶V=11∶89)as mobile phase,and the detection wavelength was 327 nm.(2)The chromatographic conditions of catalpol were as follows,mobile phase A was acetonitrile,mobile phase B was 0.1%phosphoric acid,gradient elution(from 0 to 15 min,0.8%A;from 15 to 60 min,2%A),and the detection wavelength was 210 nm.RESULTS:Chlorogenic acid showed a good linear relationship in the ranges of 4.420μg/mL to 442.0μg/mL(r=0.9998),the average recovery was 99.38%(RSD=1.26%).Catalpol showed a good linear relationship in the range of 9.760μg/mL to 488.0μg/mL(r=0.9999),the average recovery was 99.34%(RSD=1.43%).The minimum detection limits of chlorogenic acid and catalpol were 0.40 ng and 0.82 ng,and the quantitative limits were 1.20 ng and 2.44 ng,respectively.The precision RSD were 0.71%(n=6)and 0.45%(n=6),respectively.CONCLUSIONS:The method has the advantages of simple operation,good separation effect,repeatability and high accuracy,which can be used for the quality evaluation of the extract of modified Simiao Yong’an decoction.

关 键 词：加味四妙勇安汤提取物 绿原酸 梓醇 反相高效液相色谱法 

分 类 号：R932[医药卫生—生药学] R927.2[医药卫生—药学]