C型产气荚膜梭菌生产用菌种种子批的建立与鉴定  

Establishment and identification of seed batch of C.Perfringens type C for production

作　　者：刘泽文[1] 袁芳艳[1] 刘威[1] 高婷 周丹娜[1] 杨克礼[1] 郭锐[1] 李畅 田永祥[1] LIU Ze-wen;YUAN Fang-yan;LIU Wei;GAO Ting;ZHOU Dan-na;YANG Ke-li;GUO Rui;LI Chang;TIAN Yong-xiang(Institute of Animal Husbandry and Veterinary,Hubei Academy of Agricultural Sciences/Key Laboratory of Prevention and Control Agents for Animal Bacteriosis,Ministry of Agriculture and Rural Affairs/Hubei Provincial Key Laboratory of Pathogen Microbiology of Livestock and Poultry,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院畜牧兽医研究所/农业农村部畜禽细菌病防治制剂创制重点实验室/畜禽病原微生物学湖北省重点实验室,武汉430064

出　　处：《湖北农业科学》2022年第22期115-118,共4页Hubei Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0500600);湖北省自然科学基金重点类项目(群体)(2021CFA019);湖北省农业科技创新中心项目(2019-620-000-001-017);湖北省技术创新专项重大项目(2021ABA005)。

摘　　要：为建立C型产气荚膜梭菌(Clostridium perfringens)生产用菌种种子批,以C型产气荚膜梭菌C59-2株第2代基础种子作为原始种子,对原始种子通过接种改良厌气肉肝汤和肉肝胃酶消化汤连续传代至第13代,分别对基础种子1代(F1)、第5代(F5)、第10代(F10)和第13代(F13)基础种子进行纯粹检验、培养特性鉴定、生化特性鉴定、产毒素能力的测定、血清型鉴定、免疫原性鉴定。结果表明,各项指标均合格,可作为仔猪大肠杆菌病、C型产气荚膜梭菌病二联灭活疫苗的生产用菌种用于疫苗的生产,基础种子批定为F1~F10代。In order to establish the seed batch of Clostridium perfringens type C,taking the second generation basic seeds of C.perfringens C59-2 strain as the original seeds,it was continuously subcultured to 13 generations by inoculating the appropriate medium.The basic seeds of generation 1(F1),generation 5(F5),generation 10(F10)and generation 13(F13)were carried out purity test,culture characteristics,biochemical characteristics,toxin production ability,serotype and immunogenicity identification.The test results of immunogenicity identification showed that all indexes were qualified.It could be used for the production of piglet colibacillosis and Clostridium perfringens type C inactivated vaccine.The basic seed batch was F1~F10generation.

关 键 词：C型产气荚膜梭菌(Clostridium perfringens) 种子批 建立 鉴定 

分 类 号：S852[农业科学—基础兽医学]