Interactions among deep-sea mussels and their epibiotic and endosymbiotic chemoautotrophic bacteria: Insights from multi-omics analysis  

作　　者：Yi-Tao Lin Ting Xu Jack Chi-Ho Ip Yanan Sun Ling Fang Tiangang Luan Yu Zhang Pei-Yuan Qian Jian-Wen Qiu 

机构地区：[1]Department of Biology,Hong Kong Baptist University,Hong Kong SAR,China [2]Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou,Guangdong 511458,China [3]Department of Ocean Science,Hong Kong University of Science and Technology,Hong Kong SAR,China [4]Instrumental Analysis&Research Center,Sun Yat-Sen University,Guangzhou,Guangdong 510875,China [5]State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-Sen University,Guangzhou,Guangdong 510875,China [6]Institute of Environmental and Ecological Engineering,Guangdong University of Technology,Guangzhou,Guangdong 510006,China [7]College of Life Sciences and Oceanography,Shenzhen University,Shenzhen,Guangdong 518060,China

出　　处：《Zoological Research》2023年第1期106-125,共20页动物学研究（英文）

基　　金：supported by the Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(GML2019ZD0409,SMSEGL20SC02);Research Grants Council of Hong Kong(12101021);Guangdong Natural Science Foundation(2020A1515011117)。

摘　　要：Endosymbiosis with Gammaproteobacteria is fundamental for the success of bathymodioline mussels in deep-sea chemosynthesis-based ecosystems. However, the recent discovery of Campylobacteria on the gill surfaces of these mussels suggests that these host-bacterial relationships may be more complex than previously thought. Using the cold-seep mussel(Gigantidas haimaensis) as a model, we explored this hostbacterial system by assembling the host transcriptome and genomes of its epibiotic Campylobacteria and endosymbiotic Gammaproteobacteria and quantifying their gene and protein expression levels. We found that the epibiont applies a sulfur oxidizing(SOX)multienzyme complex with the acquisition of sox B from Gammaproteobacteria for energy production and switched from a reductive tricarboxylic acid (rTCA) cycle to a Calvin-Benson-Bassham(CBB)cycle for carbon assimilation. The host provides metabolic intermediates, inorganic carbon, and thiosulfate to satisfy the materials and energy requirements of the epibiont, but whether the epibiont benefits the host is unclear. The endosymbiont adopts methane oxidation and the ribulose monophosphate pathway(Ru MP) for energy production, providing the major source of energy for itself and the host. The host obtains most of its nutrients, such as lysine, glutamine, valine,isoleucine, leucine, histidine, and folate, from the endosymbiont. In addition, host pattern recognition receptors, including toll-like receptors, peptidoglycan recognition proteins, and C-type lectins, may participate in bacterial infection, maintenance, and population regulation. Overall, this study provides insights into the complex host-bacterial relationships that have enabled mussels and bacteria to thrive in deep-sea chemosynthetic ecosystems.

关 键 词：Bathymodioline CHEMOSYNTHESIS Cold seep Deep-sea adaptation SYMBIOSIS 

分 类 号：Q95[生物学—动物学]