石胡荽醇提物制备工艺优化及其不同萃取部位抗炎活性研究  

Optimization of the preparation process of ethanol extracts from Centipeda minima and study on its antiinflammatory activity of different extraction sites

作　　者：杨柳 杨艳[2] 高奇[2] 文雯[1,2] 王丽 杨小生[2] 杨娟 YANG Liu;YANG Yan;GAO Qi;WEN Wen;WANG Li;YANG Xiaosheng;YANG Juan(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Key Laboratory of Chemistry for Natural Products of Guizhou Province,Chinese Academy of Sciences,Guiyang 550014,China)

机构地区：[1]贵州中医药大学药学院,贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室,贵阳550014

出　　处：《中国药房》2023年第3期321-326,共6页China Pharmacy

基　　金：贵州省科技计划项目(No.黔科合服企[2020]4013);贵州省高等学校特色重点实验室建设项目(No.黔教合KY字[2020]018)。

摘　　要：目的 优化石胡荽醇提物制备工艺,并考察其不同萃取部位的抗炎活性。方法 采用单因素实验和响应面法,以石胡荽总三萜提取率为评价指标,考察乙醇体积分数、提取时间、料液比、提取次数4个因素对总三萜醇提物加热回流提取工艺的影响,优化最佳工艺,并进行验证实验。以地塞米松为阳性对照药物,比较石胡荽不同萃取部位(石油醚部位、乙酸乙酯部位、正丁醇部位、水部位)对脂多糖诱导小鼠单核巨噬细胞RAW 264.7一氧化氮(NO)生成的影响并计算其半数抑制浓度(IC50)。结果 石胡荽总三萜醇提物的最优提取工艺为乙醇体积分数70%、料液比1∶40(g/mL)、提取时间2.0 h、提取次数3次。3次验证实验表明,石胡荽总三萜的平均提取率为1.134%,与预测值的相对误差为0.02%。石胡荽的石油醚部位、乙酸乙酯部位可不同程度地降低由脂多糖诱导的RAW 264.7细胞的NO生成,其对NO生成的IC50分别为2.44、2.22μg/mL,均低于阳性对照药物地塞米松(7.65μg/mL)。结论 优化后的石胡荽醇提物的制备工艺稳定可行,其石油醚部位、乙酸乙酯部位具有较好的抗炎活性。OBJECTIVE To optimize the preparation technology of ethanol extracts from Centipeda minima, and investigate the anti-inflammatory activities of different extraction sites. METHODS Single factor test and response surface methodology were adopted to investigate the effects of ethanol volume fraction, extraction time, solid-liquid ratio and extraction times on the heating reflux extraction technology of total triterpenoids ethanol extract using the extraction rate of total triterpenoids of C. minima as indexes, optimize the extraction technology and carry out validation. Using dexamethasone as positive control drug, the effects of different extraction sites of C. minima(petroleum ether part, ethyl acetate part, n-butanol part, water part) on nitric oxide(NO) production in mononuclear macrophage RAW 264.7 cells of mice induced by lipopolysaccharide(LPS) were compared;the half inhibitory concentration(IC50) was calculated. RESULTS The optimal extraction technology of total triterpenoids ethanol extracts of C. minima was as follows: ethanol volume fraction of 70%, solid-liquid ratio of 1∶40(g/mL), extraction time of 2.0 h and extraction times of 3 times. The 3 times of validation tests showed that average extraction rate of total triterpenoids of C. minima was 1.134%, relative error of which with the predicted value was 0.02%. The petroleum ether part and ethyl acetate part of C.minima could inhibit the generation of NO in RAW 264.7 cells induced by lipopolysaccharide to different degrees. IC50 values of NO production were 2.44 μg/mL and 2.22 μg/mL, respectively, and both of them were lower than those of positive control drug dexamethasone(7.65 μg/mL). CONCLUSIONS The optimized preparation process of ethanol extracts from C. minima is stability and feasibility. The petroleum ether part and ethyl acetate part have obvious anti-inflammatory effects.

关 键 词：石胡荽 总三萜 响应面法 制备工艺 抗炎活性 

分 类 号：R917[医药卫生—药物分析学] R284.2[医药卫生—药学]