牦牛源富硒鼠李糖乳杆菌的优化培养  

Optimized culture of yak-derived selenium-rich Lactobacillus rhamnosus

作　　者：孙建春 吴征王 邹雨婷 石斌[3] 吴庆侠[4] 董海龙[4] 田发益[4] SUN Jian-chun;WU Zheng-wang;ZOU Yu-ting;SHI Bin;WU Qing-xia;DONG Hai-long;TIAN Fa-yi(Quality and Safety Inspection and Testing Center for Agricultural and Animal Products of Tibet Autonomous Region,Lhasa 850013,China;Quxian County Animal Disease Prevention and Control Center,Dazhou 635200,Sichuan,China;Animal Husbandry and Veterinary Research Institute,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850004,China;Tibet Agricultural and Animal Husbandry University,Linzhi 860000,Tibet,China)

机构地区：[1]西藏自治区农畜产品质量安全检验检测中心,拉萨850013 [2]渠县动物疾病预防控制中心,四川达州635200 [3]西藏自治区农牧科学院畜牧兽医研究所,拉萨850004 [4]西藏农牧学院,西藏林芝860000

出　　处：《湖北农业科学》2022年第24期53-55,59,共4页Hubei Agricultural Sciences

基　　金：国家科技部科技支撑计划项目(2016YFC0502004)。

摘　　要：为了生产出硒量较高的牦牛源鼠李糖乳杆菌,采用含不同浓度亚硒酸钠的液体培养基、不同培养温度、不同加硒时间设计培养方案。结果表明,最佳培养条件是培养温度38℃、亚硒酸钠浓度12μg/mL、加硒时间第6 h,培养24 h,转化率为50.34%、富硒量高达5658.256μg/g。本研究为生产富硒乳酸菌产品提供了较好的依据。In order to produce Lactobacillus rhamnosus of yak origin with high selenium content,a culture protocol was designed using the liquid medium containing different concentrations of sodium selenite,different incubation temperatures and different selenium addition times.The results showed that the best culture conditions were the incubation temperature of 38℃,sodium selenite concentration of 12μg/mL,selenium addition time of the 6th h,and 24 h incubation,the conversion rate was 50.34%and the selenium enrichment was up to 5658.256μg/g.This study provided a better basis for the production of selenium-enriched lactic acid bacteria products.

关 键 词：鼠李糖乳杆菌 富硒 牦牛源 

分 类 号：S854-04[农业科学—临床兽医学]