基于正交设计的西洋梨‘Conference’愈伤组织诱导研究  

Study on inducing callus of pear(Pyrus communis L.‘Conference’)by orthogonal array-based method

作　　者：杨立[1] 伍涛[1] 刘政[1] 程寅胜 李谢雨 聂显双 秦仲麒[1] YANG Li;WU Tao;LIU Zheng;CHENG Yin-sheng;LI Xie-yu;NIE Xian-shuang;QIN Zhong-qi(Institute of Fruit Tree Tea/Pear Engineering Technology Research Center of Hubei Province,Hubei Academy of Agricultural Sciences,Wuhan 430070,China)

机构地区：[1]湖北省农业科学院果树茶叶研究所/湖北省梨工程技术研究中心,武汉430070

出　　处：《湖北农业科学》2022年第24期160-162,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31801819);湖北省自然科学基金项目(2018CFB285);湖北省农业科学院青年科学基金项目(2020NKYJJ12);湖北省农业科技创新中心重大项目(2020-620-000-002-05)。

摘　　要：以西洋梨‘Conference’为材料诱导愈伤组织,采用L9(34)正交设计法,探讨各因子对‘Conference’梨愈伤组织诱导效果的影响。结果表明,各因素对愈伤组织诱导率的影响表现为TDZ浓度>外植体类型>IBA浓度,TDZ、IBA浓度对试验结果的影响呈显著水平(P<0.05),不同水平间也差异显著。最优组合为A1B3C3,即以茎段为外植体,培养基和植物生长调节剂组合为MS+2.0 mg/L TDZ+0.5 mg/L IBA中暗培养35 d,诱导率可达100%。Pyrus communisL.‘Conference’was used as material to induce callus,and L9(3~4)orthogonal design was used to explore the effects of various factors on callus induction of‘Conference’pear.The results showed that the order of influence of each factor on callus induction:TDZ concentration>explants type>IBA concentration,and the influence of TDZ and IBA on the experimental results was significant(P<0.05),in addition,there were significant differences among different levels.A1B3C3proved to be the best combination,stem segments were used as explants,MS+2.0 mg/L TDZ+0.5 mg/L IBA medium and dark culture for 35 days were adopted,and the induction rate was 100%.

关 键 词：‘Conference’梨 组织培养 愈伤组织 正交设计 

分 类 号：S661[农业科学—果树学]