基于加权基因共表达网络分析探究桑树光合相关基因的调控模式  

Analysis of the regulation patterns of photosynthesis-related genes in mulberry based on WGCNA

作　　者：李勇[1] 黄锦 于翠[1] 莫荣利[1] 朱志贤 董朝霞 胡兴明[1] 邓文[1] LI Yong;HUANG Jin;YU Cui;MO Rong-li;ZHU Zhi-xian;DONG Chao-xia;HU Xing-ming;DENG Wen(Cash Crops Research Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院经济作物研究所,武汉430064

出　　处：《湖北农业科学》2022年第24期163-169,共7页Hubei Agricultural Sciences

基　　金：湖北省重点研发计划项目(2022BBA0065);国家现代农业产业技术体系建设专项项目(CARS-18)。

摘　　要：基于前期桑树转录组数据和光合表型数据,通过加权基因共表达网络分析(WGCNA)共获得10个基因模块与表型关联,筛选到MEmagenta、MEgreen、MEyellow、MEpurple和MEblack光合相关基因模块。GO和KEGG分析显示,DEGs显著富集在光合相关酶活性、碳代谢和固碳等途径,筛选到12个与光合作用高度相关的基因。通过RNA-seq结合WGCNA分析,筛选了3个光合关键基因,即光系统Q(B)蛋白L484_000029基因、核糖二磷酸羧化酶L484_000836基因、核糖二磷酸羧化酶/加氧酶激活酶2 L484_025354基因。该结果为进一步研究桑树光合作用调控机理提供支持。Based on the previous mulberry transcriptome data and photosynthetic phenotype data,through WGCNA analysis,10 gene modules associated with the phenotype were obtained,and screenedthe photosynthetic-related gene modules of MEmagenta,MEgreen,MEyellow,MEpurple,and MEblack were screened.GO and KEGG analysis showed that DEGs were significantly enriched in the activities of photosynthesis-related enzymes,carbon metabolism and carbon fixation.12 genes highly related to photosynthesis were screened.Finally,through RNA-seq combined with WGCNA analysis,three key photosynthetic genes were selected,namely,photosystem Q(B)protein L484_000029 gene,ribose bisphosphate carboxylase L484_000836 gene,and ribose bisphosphate carboxylase/oxygenase activator 2 L484_025354 gene.The results provided support for further research on the regulation mechanism of mulberry photosynthesis.

关 键 词：加权基因共表达网络分析 桑树 光合相关基因 

分 类 号：S888.2[农业科学—特种经济动物饲养]