两种培养条件下重组杆状病毒表达蓝舌病病毒VP7蛋白的丰度研究  

Study on the abundance of VP7 protein of Bluetongue virus expressed by recombinant Baculovirus under two culture conditions

作　　者：陈朝林 韩佃刚 杨云庆 张冲 李静 罗倩敏 尹尚莲 董仙兰 李凌枫 师亚玲 艾军 信吉阁 CHEN Chao-lin;HAN Dian-gang;YANG Yun-qing;ZHANG Chong;LI Jing;LUO Qian-min;YIN Shang-lian;DONG Xian-lan;LI Ling-feng;SHI Ya-ling;AI Jun;XIN Ji-ge(College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China;Kunming Customs District P.R.China,Kunming 650228,China)

机构地区：[1]云南农业大学动物医学院,昆明650201 [2]中华人民共和国昆明海关,昆明650228

出　　处：《湖北农业科学》2022年第23期117-120,共4页Hubei Agricultural Sciences

基　　金：国家质检总局科技计划项目(2011IK018);海关总署科研项目(2019HK032)。

摘　　要：为了在重组杆状病毒上获得高丰度表达的蓝舌病病毒(BTV)VP7蛋白,利用细胞贴壁培养和摇瓶培养法培养重组杆状病毒,培养5 d,每天采集培养液,用ELISA方法检测其中BTV VP7蛋白的表达量,用SPSS软件对数据进行统计分析。结果显示,贴壁培养条件下BTV VP7蛋白的表达量显著高于摇瓶培养,两种培养条件下BTV VP7蛋白均在第三天时表达量最高,之后开始下降。研究表明用杆状病毒昆虫细胞系统表达BTV VP7蛋白时更适合用贴壁培养方法进行大规模表达。In order to obtain Bluetongue virus(BTV)VP7 protein with high abundance expression on the recombinant Baculovirus,the recombinant Baculovirus was cultured by cell attachment culture and shake flask culture.After 5 days of culture,the culture medium was collected every day.The expression of BTV VP7 protein was detected by ELISA,and the data were statistically analyzed by SPSS software.The results showed that the expression of BTV VP7 protein in adherent culture was significantly higher than that in shake flask culture.The expression of BTV VP7 protein in both culture conditions was the highest on the third day,and then began to decline.The results showed that the Baculovirus insect cell system was more suitable for large-scale expression of BTV VP7 protein by adherent culture.

关 键 词：Sf9昆虫细胞 蓝舌病病毒VP7蛋白 贴壁培养 摇瓶培养 

分 类 号：S852.65[农业科学—基础兽医学]