不同培养基对水稻花药培养力的影响  

Effects of different media on anther culture of rice

作　　者：查中萍[1] 郭英[1] 殷得所[1] 胡建林 郑兴飞 董华林[1] 刘艳芳 王红波[1] 薛莲 徐得泽 ZHA Zhong-ping;GUO Ying;YIN De-suo;HU Jian-lin;ZHENG Xing-fei;DONG Hua-lin;LIU Yan-fang;WANG Hong-bo;XUE Lian;XU De-ze(Key Laboratory of Crop Molecular Breeding,Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement/Food Crops Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;Hubei Hongshan Laboratory,Wuhan 430070,China;Hubei Academy of Scientific and Technical Information,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院粮食作物研究所/农业农村部作物分子育种重点实验室/粮食作物种质创新与遗传改良湖北省重点实验室,武汉430064 [2]湖北洪山实验室,武汉430070 [3]湖北省科技信息研究院,武汉430064

出　　处：《湖北农业科学》2022年第23期202-205,共4页Hubei Agricultural Sciences

基　　金：湖北省重点研发计划项目(2020BBB053);国家水稻产业技术体系“武汉综合试验站”项目;湖北省重大科技专项(2021ABA011);湖北省重点研发计划(2020BBA031);湖北省农业科技创新中心资助项目(2020-620-000-002-01);湖北省农业科学院领军人才项目。

摘　　要：以不同籼粳成分的水稻(Oryza sativa L.)为试验材料,研究不同基本培养基[N6、合5、SK3、M8以及改良的M8(GM8)]对愈伤组织诱导效率的影响,筛选水稻花药培养最适宜的诱导培养基。结果表明,最佳诱导基本培养基为GM8;再以GM8为基本培养基,以HB568(粳稻)、HB570(糯稻)、HB613(籼稻)及21ah36(野生稻)为试验材料,比较添加单一、复合生长调节剂的诱导培养基对水稻花药培养的效果。结果表明,添加复合生长调节剂的处理可以提高花药培养力,即最佳水稻花药培养力的培养基为GM8+1.0 mg/L KT+2.0 mg/L 2,4-D+3.0 mg/L NAA。The effects of different basic media[N6,He5,SK3,M8 and modified M8(GM8)]on callus induction efficiency of rice(Oryza sativa L.)with different indica and japonica components were studied to select the most suitable induction medium for rice anther culture.The results showed that the optimal induction medium was GM8;then GM8 was used as the basic medium,HB568(japonica rice),HB570(glutinous rice),HB613(indica rice)and 21ah36(wild rice)were used as experimental materials to compare the effects of single and compound growth regulators on rice anther culture.The results showed that the addition of compound growth regulators could improve the anther culture ability,and the best medium for rice anther culture was GM8+1.0 mg/L KT+2.0 mg/L2,4-D+3.0 mg/L NAA.

关 键 词：水稻(Oryza sativa L.) 花药培养 诱导培养基 花药培养力 

分 类 号：S511[农业科学—作物学]