马源肠道芽孢杆菌的16S rDNA序列测定及系统进化分析  

Sequence determination and phylogenetic analysis of 16S rDNA of Bacillus from intestinal tract of horse

作　　者：向双云[1] 周珍辉[1] XIANG Shuang-yun;ZHOU Zhen-hui

机构地区：[1]北京农业职业学院畜牧兽医系,北京102442

出　　处：《饲料研究》2022年第22期102-105,共4页Feed Research

基　　金：北京市特色高水平骨干专业群项目-动物医学专业群项目-技术平台与社会服务建设项目“马肠道微生物中芽孢杆菌的分离与鉴定(项目编号:PXM2020-157102-000060-15)”。

摘　　要：研究旨在对马源肠道芽孢杆菌进行16S rDNA测序和系统进化分析。采集5匹成年马的新鲜粪便,经划线分离、纯化镜检、生化鉴定后获得5株菌,分别命名为QM、BM、Y1M、Y2M、Z2M,采用16S rDNA扩增通用引物,进行序列测定,测序结果与GenBank DNA数据库的序列比对,并构建进化树。结果显示:QM、BM、Y1M均为蜡样芽孢杆菌,Y2M为解淀粉芽孢杆菌,Z2M为赖氨酸芽孢杆菌。研究表明,马肠道内含有丰富的芽孢杆菌,可为马源益生菌提供天然菌种,促进马属动物肠道健康。The study was to conduct 16S rDNA sequencing and phylogenetic analysis of enterobacteriaceae of equine origin. The fresh feces of five adult horses were collected, and the five strains were obtained after stripe separation,purification microscopy and biochemical identification. The strains were named QM, BM, Y1M, Y2M, Z2M, respectively.The 16S r DNA amplification universal primers were used for sequence determination. The sequencing results were compared with GenBank DNA database, and the evolutionary tree was constructed. The results showed that QM, BM and Y1M were all Bacillus cereus, Y2M was Bacillus amylolivier and Z2M was Bacillus lysine. The study indicates that horse intestinal tract is rich in bacillus, which can provide natural strains for horse probiotics and promote intestinal health of horse.

关 键 词：马源肠道 芽孢杆菌 分离 纯化 生化鉴定 16S rDNA序列测定 

分 类 号：S816.7[农业科学—饲料科学]