查吾拉牦牛线粒体全基因组序列及系统发育分析  

Sequence and Phylogenetic Analysis of the Complete Mitochondrial Genome in Chawula Yak

作　　者：张强 任稳稳 次旦央吉 达瓦央拉 巴桑旺堆 陈晓英[1,2] 平措占堆 姜辉[1,2] ZHANG Qiang;REN Wenwen;CIDAN Yangji;DAWA Yangla;BASANG Wangdui;CHEN Xiaoying;PINGCUO Zhandui;JIANG Hui(State Key Laboratory of Highland Barley and Yak Germplasm Resources and Genetic Improvement,Lhasa850000,China;Institute of Animal Science and Veterinary,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa850009,China;Key Laboratory of Yak Breeding Engineering of Gansu Province,Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou730050,China;Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau,Ministry of Agriculture and Rural Affairs,Lanzhou730050,China)

机构地区：[1]省部共建青稞和牦牛种质资源与遗传改良国家重点实验室,拉萨850000 [2]西藏农牧科学院畜牧兽医研究所,拉萨850009 [3]中国农业科学院兰州畜牧与兽药研究所,甘肃省牦牛繁育工程重点实验室,兰州730050 [4]农业农村部青藏高原畜禽遗传育种重点实验室,兰州730050

出　　处：《中国草食动物科学》2023年第1期25-28,共4页China Herbivore Science

基　　金：西藏自治区重大科技项目(QYXTZX-NQ2022-01);国家肉牛产业技术与系统(CARS-37);农业资源调查、保护与利用项目。

摘　　要：为了解查吾拉牦牛(Bos grunniens)线粒体全基因组结构,对查吾拉牦牛线粒体基因组进行了PCR扩增并测序、组装及注释。结果表明,查吾拉牦牛完整的线粒体DNA为环状分子,全长16 323 bp,其核苷酸组成分别为:A占33.71%,T占27.30%,C占25.78%,G占13.21%,A+T含量为61.01%。总体组成包括22个tRNA基因,2个rRNA基因,13个蛋白编码基因(ND1~ND6、ND4L、COX1~COX3、ATP6、ATP8和CYTB),以及1个非编码控制区(D-loop)。ND6和8个tRNA基因(tRNA-Ser、tRNA-Pro、t RNA-Glu、t RNA-Tyr、tRNA-Cys、tRNA-Asn、tRNA-Ala和tRNAGln)均编码在轻链上,其余基因编码在重链上。系统发育分析结果表明,查吾拉牦牛与娘亚牦牛亲缘关系较近。综上所述,查吾拉牦牛线粒体基因组全序列可用于进一步研究其起源进化和育种改良。In order to understand the complete mitochondrial genome structure of Chawula yak,the mitochondrial genome of Chawula yak was amplified by PCR,sequenced,assembled,and annotated. The results showed that the complete mitochondrial DNA of Chawula yak was a circular molecule with the length of 16 323 bp,in which A accounted for 33.71%,T for 27.30%,C for25.78%,and G for 13.21%,the content of A+T was 61.01%. The overall composition included 22 tRNA genes,2 rRNA genes,13protein-coding genes(ND1-ND6,ND4L,Cox1-CoX3,ATP6,ATP8 and CYTB),and a non-coding control region(D-loop).ND6 and 8 tRNAs(tRNA-Ser,t RNA-Pro,tRNA-Glu,t RNA-Tyr,t RNA-Cys,tRNA-Asn,tRNA-Ala and tRNA-Gln)genes were encoded on the light chain,while the other genes were encoded on the heavy chain. Phylogenetic analysis showed that Chawula yak was closely related to Niangya yak. In conclusion,the complete mitochondrial genome sequence of Chawula yak can be used to further study its origin,evolution and breeding improvement.

关 键 词：查吾拉牦牛 线粒体基因组 系统发育分析 

分 类 号：S823.2[农业科学—畜牧学]