番茄bZIP转录因子响应低温胁迫的功能研究  

Function of Solanum lycopersicum bZIP transcription factor in response to low temperature stress

作　　者：徐洪伟[1,2] 金妍 刘美琦 周晓馥[1,2] XU Hong-wei;JIN Yan;LIU Mei-qi;ZHOU Xiao-fu(Collage of Life Science,Jilin Normal University,Siping 136000,China;x0E䥺Symbol`@@0x0F 2.Jilin Provincial Key Laboratory of Plant Resource Science and Green Production,Jilin Normal University,Siping 136000,China)

机构地区：[1]吉林师范大学生命科学学院,吉林四平136000 [2]吉林师范大学吉林省植物资源科学与绿色生产重点实验室,吉林四平136000

出　　处：《吉林师范大学学报（自然科学版）》2023年第1期112-122,共11页Journal of Jilin Normal University:Natural Science Edition

基　　金：吉林省科技发展计划项目(20190301059NY);中央指导地方科技发展资金吉林省基础研究专项资助项目(202002016JC)。

摘　　要：通过生物信息学方法鉴定番茄基因组上的bZIP转录因子(SlbZIP),为研究番茄bZIP转录因子提供相关信息以及为低温胁迫下的候选基因预测提供参考.通过Pfam下载bZIP隐马尔科夫模型bZIP_1(PF00170),利用HMMER 3.0鉴定番茄bZIP转录因子,并从Plant PFDB数据库中获取其理化性质等信息.使用Clustal W、MEGA11.0、TBtools和MEME等在线软件对其蛋白序列进行生物信息学分析.通过与拟南芥的bZIP转录因子家族进行系统进化树分析,将番茄bZIP分为13个亚族(A-I和S、X、Z).染色体定位分析显示,番茄bZIP转录因子不平均分布在12条染色体上.保守基序和进化树分析表明,同一亚族同一分支的转录因子基序类型大致相同,预示同一分支的转录因子功能可能相似.未知功能的番茄bZIP转录因子蛋白二级结构和三级结构预测表明,番茄bZIP转录因子蛋白以无规则卷曲和α螺旋为主,且占比较大,此类结构对番茄bZIP转录因子蛋白功能的发挥有重要作用.未知功能的番茄bZIP转录因子与拟南芥的进化树表明,10个番茄bZIP转录因子与拟南芥中能够抵御低温胁迫的转录因子亲缘关系较近,推测10个番茄bZIP转录因子可能抵御低温胁迫.Identification of bZIP transcription factors(SlbZIP)on the tomato genome by bioinformatic methods was used to provide relevant information for the study of tomato bZIP transcription factors and for candidate gene prediction under low temperature stress.The bZIP Hidden Markov Model bZIP_1(PF00170)was downloaded via Pfam,the tomato bZIP transcription factor was identified using HMMER 3.0,and information on its physicochemical properties was obtained from the Plant PFDB database.Their protein sequences were bioinformatically analyzed using online software such as Clustal W,MEGA11.0,TBtools and MEME.Tomato bZIP was classified into 13 subfamilies(A-I and S,X,Z)by phylogenetic tree analysis with the bZIP transcription factor family of Arabidopsis thaliana.Chromosomal localization analysis revealed that tomato bZIP transcription factors were not equally distributed on 12 chromosomes.Conservative motif and evolutionary tree analyses showed that transcription factors of the same subfamily in the same branch have approximately the same motif type,predicting that transcription factors of the same branch may function similarly.The secondary and tertiary structures of tomato bZIP transcription factor proteins of unknown function were predicted to be predominantly irregularly coiled andα-helices,and such structures play an important role in the function of tomato bZIP transcription factor proteins.The evolutionary tree of tomato bZIP transcription factors of unknown function with Arabidopsis showed that 10 of them are closely related to transcription factors capable of resisting low temperature stress in Arabidopsis,and it is hypothesized that 10 tomato bZIP transcription factors may resist low temperature stress.

关 键 词：番茄 bZIP转录因子 低温胁迫 

分 类 号：Q943.2[生物学—植物学]