Plasma metabolomics study of Buyang Huanwu Decoction improving learning andmemory in D-gal model mice based on GC-MS  

作　　者：YU Ming-hui XUE Ao ZHAO De-ping XU Yan-ming XUE Hui JIANG Jing SUN Hui-feng ZHANG Ning 

机构地区：[1]College of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China [2]College of Jiamusi,Heilongjiang University of Chinese Medicine,Jiamusi 154007,China [3]Heilongjiang Provincial Institute of Drug Inspection,the Research Institute for Biosafety Inspection,Harbin 150088,China

出　　处：《Journal of Hainan Medical University》2022年第22期1-7,共7页海南医学院学报（英文版）

基　　金：National Natural Science Foundation of China(No.82174007);Talent Training Project Supported by the Central Government for the Reform and Development of Local Universities;Research Project of Heilongjiang Provincial Health Commission(No.2020-303);National Natural Science Foundation of China(No.82003975);Fund Project of Heilongjiang University of Traditional Chinese Medicine(No.2018RCD19,2018pt04)。

摘　　要：Objective:To study the protective effect of Buyang Huanwu Decoction on the learning and memory ability of D-gal induced aging mice using GC-MS metabolomics method.Methods:Twenty-four three-month-old kunming animals were selected as experimental samples and randomly divided into control group,model group and Buyang Huanwu Decoction according to their body weight.The memory level of experimental animals was detected by novel body recognition test,and the neuron structure of experimental animals was detected by HE staining.The plasma of each group of experimental animals was quantitatively analyzed by gas chromatography-mass spectrometry,and the important metabolites and main metabolic pathways in the process of pathological changes were traced by using plasma metabolism.Results:In HE staining,compared with blank group,hippocampal neurons in model group were disordered and morphologically abnormal.Compared with the model group,the hippocampal neurons in Buyang Huanwu Decoction group arranged regularly and had normal morphology.Compared with blank group,the index of new object recognition in model group was significantly decreased(P<0.05);Compared with model group,the new object recognition index of Buyang Huanwu Decoction group was significantly increased(P<0.05).Five different metabolites of AD were identified by GC-MS,which were L-pyroglutamate,lysine,pyrophosphoric acid,creatinine andα-lactose.Conclusion:Buyang Huanwu Decoction has a significant effect on D-gal model mice,and can effectively improve their learning and memory level.The mechanism may be related to glutathione metabolism and aminophyl biosynthesis.

关 键 词：Buyang Huanwu Decoction Alzheimer's disease GC-MS Plasma metabolomics 

分 类 号：O657.63[理学—分析化学] R285.5[理学—化学]