妊娠糖尿病患者尿液8-oxo-Gsn表达水平及其对妊娠结局预测价值研究  

Urinary 8-oxo-Gsn Expression Level in Patients with Gestational Diabetes Mellitus and Its Predictive Value for Pregnancy Outcome

作　　者：李婧 程涵芬 LI Jing;CHENG Han-fen(Department of Clinical Laboratory,Hanchuan Hospital of Traditional Chinese Medicine,Hubei Hanchuan 431600,China)

机构地区：[1]汉川市中医医院检验科,湖北汉川431600

出　　处：《现代检验医学杂志》2023年第1期135-139,共5页Journal of Modern Laboratory Medicine

摘　　要：目的探讨妊娠糖尿病(gestational diabetes mellitus,GDM)患者尿液RNA氧化标志物8G-氧化鸟苷(8-oxo-7,8-dihydroguanosine,8-oxo-Gsn)的水平及与妊娠结局的关系。方法选取2018年2月~2021年2月汉川市中医医院收治的106例GDM患者为观察组,根据妊娠结局情况分为妊娠结局良好组和妊娠结局不良组。另选取同期进行体检的90例健康孕妇为对照组。收集所有孕妇的临床资料并进行比较。采用同位素稀释高效液相-串联质谱法(isotope dilution high performance liquid phase-tandem mass spectrometry,ID-LC-MS/MS)分析尿液中8-oxo-Gsn水平。采用ROC曲线评估8-oxo-Gsn对GDM患者不良妊娠结局的评估价值。多因素回归分析影响GDM患者不良妊娠结局的危险因素。结果与对照组相比,观察组尿液8-oxo-Gsn水平(3.89±0.63μmol/mol vs 2.26±0.47μmol/mol)及不良妊娠结局的发生率(29.25%vs 11.11%)明显升高,差异有统计学意义(t=20.225,χ^(2)=9.676,均P<0.05)。与妊娠结局良好组相比,妊娠结局不良组患者尿液8-oxo-Gsn水平(4.67±0.77μmol/mol vs 3.56±0.57μmol/mol)升高,差异有统计学意义(t=8.197,P<0.05)。ROC曲线分析结果显示,8-oxo-Gsn预测GDM患者不良妊娠结局的曲线下面积为0.847,截断值为4.049μmol/mol,敏感度和特异度分别为77.40%,81.30%。HOMA-IR[OR(95%CI):4.726(2.027~11.021)],8-oxo-Gsn[OR(95%CI):5.591(2.239~13.964)]是GDM患者发生不良妊娠结局的独立危险因素(均P<0.05)。结论GDM患者尿液8-oxo-Gsn水平升高,与患者不良妊娠结局有关,且对不良妊娠结局具有较优的预测价值。Objective To investigate the level of urine RNA oxidation marker 8-oxo-7 and 8-dihydroguanosine(8-oxo-Gsn)in patients with gestational diabetes(GDM),and its relationship with pregnancy outcome.Methods A total of 106 GDM patients admitted to Hanchuan Hospital of Traditional Chinese Medicine from February 2018 to February 2021 were selected as the observation group,and divided into good pregnancy outcome group and bad pregnancy outcome group according to the pregnancy outcome.Another 90 healthy pregnant women who underwent physical examination during the same period were selected as the control group.The clinical data of all pregnant women were collected and compared.The level of 8-oxo-Gsn in urine of all pregnant women was analyzed by isotope dilution high performance liquid phase-tandem mass spectrometry(ID-LCMS/MS).ROC curve was used to analyze the evaluation value of 8-oxo-Gsn for adverse pregnancy outcomes in GDM patients.Multivariate regression analysis affected risk factors for adverse pregnancy outcomes in GDM patients.Results Compared with the control group,the urine 8-oxo-Gsn level(3.89±0.63μmol/mol vs 2.26±0.47μmol/mol)and the incidence of adverse pregnancy outcomes(29.25%vs 11.11%)in the observation group were increased,and the differences were statistically significant(t=20.225,χ^(2)=9.676,all P<0.05).Compared with the good pregnancy outcome group,the urine 8-oxo-Gsn level(4.67±0.77μmol/mol vs 3.56±0.57μmol/mol)of pregnant women in the poor pregnancy outcome group was higher,and the difference was statistically significant(t=8.197,P<0.05).ROC curve analysis results showed that 8-oxo-Gsn predicted the area under the curve of pregnancy outcome in GDM patients was 0.847,the cut-off value was 4.049μmol/mol,the sensitivity and the specificity were 77.40%,81.30%,respectively.HOMA-IR[OR(95%CI):4.726(2.027~11.021)],8-oxo-Gsn[OR(95%CI):5.591(2.239~13.964)]were independent risk factors for adverse pregnancy outcomes in GDM patients(all P<0.05).Conclusion Increased urine 8-oxo-Gsn level in GDM patients

关 键 词：8G-氧化鸟苷 妊娠糖尿病 妊娠结局 

分 类 号：R714.256[医药卫生—妇产科学] R392.11[医药卫生—临床医学]