液相色谱串联质谱法测定香蕉中三唑类农药  

Determination of Triazole Pesticide Residues in Bananas by UPLC-MS/MS

作　　者：张月[1] 黄海珠[1] 韩丙军[1] ZHANG Yue;HUANG Haizhu;HAN Bingjun(Analysis and Testing Center,Chinese Academy of Tropical Agricultural Sciences/Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables,Haikou,Hainan 571101)

机构地区：[1]中国热带农业科学院分析测试中心/海南省热带果蔬产品质量安全重点实验室,海南海口571101

出　　处：《热带农业工程》2022年第6期130-133,共4页Tropical Agricultural Engineering

基　　金：海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(No.2019RC263);财政部和农业农村部国家现代农业产业技术体系资助(No.CARS-31)。

摘　　要：建立液质联用法测定香蕉中三唑类农药残留(戊唑醇、氟环唑、氟硅唑、丙环唑、腈菌唑)的分析方法。结果发现,样品经提取,PSA净化,以水和甲醇为流动相进行梯度洗脱,基质匹配曲线外标法定量,5种三唑类农药在2.0~100.0μg/L呈良好线性关系,在0.005、0.01和0.1 mg/kg 3水平下,5种农药平均回收率为90%~118%,相对标准偏差(n=5)为0.8%~8.8%,5种农药均存在不用强度的基质效应。该方法灵敏度及准确度良好,能满足香蕉中5种三唑类杀菌剂残留检测的要求。To develop a method for triazole pesticide residues(pentazole alcohol, flucyclozole, flucysilazole, propicyclozole, nitrile) in bananas by UPLC-MS/MS. Samples were extracted by acetonitrile, purified by PSA with gradient elution using mobile phase made up of water and methanol, quantified by substrate matched curve. 5 kinds of triazole pesticides showed good linearity in the concentration range of 2.0~100.0 μg/L, the average recovery rates at three spiked levels of 0.005,0.01 and 0.1 mg/kg ranged from 90%to 118%. The relative standard deviations(RSDs)(n=5) were 0.8%~8.8%, and during the process, all the 5pesticides had matrix effects of different intensities. So far, the method is sensitive and accurate, which could meet the requirements for the determination of 5 kinds of triazole pesticide residues in banana.

关 键 词：三唑类农药 香蕉 液质联用仪 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]