遮荫处理对反瓣虾脊兰叶绿素荧光特性的影响  

Effects of Shading Treatment on Chlorophyll Fluorescence Characteristics of Calanthe Reflexa Leaves

作　　者：陈鹏英 杨婵 张清舒 周帅 杨依康 罗建 CHEN Pengying;YANG Chan;ZHANG Qingshu;ZHOU Shuai;YANG Yikang;LUO Jian(Research Institute of Tibet Plateau Ecology,Tibet Agriculture&Animal Husbandry University,Key Laboratory of Forest Ecology of Tibet Plateau,Ministry of Education,National Key Station of Field Scientific Observation&Experiment of Alpine Forest Ecology System in Nyingchi Tibet;Key Laboratory of Alpine Vegetation Ecological Security;Plant Sciences College,Tibet Agriculture&Animal Husbandry University;Resource&Environment College,Tibet Agriculture&Animal Husbandry University,Nyingchi Tibet,860000,China)

机构地区：[1]西藏农牧学院高原生态研究所,西藏高原森林生态教育部重点实验室,西藏林芝高山森林生态系统国家野外科学观测研究站 [2]西藏高寒植被生态安全重点实验室 [3]西藏农牧学院植物科学学院 [4]西藏农牧学院资源与环境学院,西藏林芝860000

出　　处：《高原农业》2022年第6期585-592,共8页Journal of Plateau Agriculture

基　　金：第二次青藏高原综合科学考察研究(2019QZKK0502);西藏自治区高寒植被生态安全重点实验室(STAQ-2021Y-8);中央引导地方项目(XZ202101YD0016C)。

摘　　要：以野外收集并定植于塑料花盆中的反瓣虾脊兰种质资源为试验材料,在林芝自然温度下,使用分光光度计(DR-6000)测定其叶绿素(Chl)和类胡萝卜素(Car)含量;叶绿素荧光参数及光响应曲线利用PAM-2500便携式荧光仪测定。研究了不同光强对其叶绿素a、b,叶绿素总量[Chlb(a+b)]类胡萝卜素,叶绿素荧光参数及光响应曲线的影响,研究表明:4个光强条件下叶绿素a(Chla)、叶绿素b(Chlb)、类胡萝卜素(Car)以及叶绿素总量[Chlb(a+b)]差异均达到显著水平,“中光”的光合色素含量显著高于其他3个条件;“低光”、“中光”和“高光”的Fo(初始荧光)、Fm(最大荧光)、Fv(可变荧光)均显著性高于对照组,说明对照组的植株的光合生理功能较弱;快速光响应曲线测试发现随PAR增加4个光强条件下的电子传递速率(rETR)均迅速升高,最大电子传输效率(rETRmax)、半饱和光强(Ik)和初始斜率(α)均表现出“低光”>“中光”>“高光”>对照组的趋势,说明光化学效率最高的是“低光”条件;4个光强条件下的有效光化学效率和实际光化学效率最高的均是“低光”。The contents of chlorophyll(Chl)and carotenoid(Car)were determined by spectrophotometer(DR-6000)at natural temperature in Nyingchi,and the experimental materials,Calanthe reflexa,were collected in the field and planted in plastic pots.The chlorophyll fluorescence parameters and the light response curves were measured by PAM-2500 portable fluorimeter.The effects of different light intensity on chlorophyll A,B,total chlorophyll[Chlb(A+B)],carotenoid,chlorophyll fluorescence parameters and light response curve were studied.The research showed that chlorophyll A(Chla),chlorophyll B(Chlb),carotenoid(Car)and total chlorophyll[Chlb(A+B)]had significant differences under four light intensities,and the photosynthetic pigment content of’medium light’was significantly higher than that of the other three conditions.Fo(initial fluorescence),Fm(maximum fluorescence)and Fv(variable fluorescence)of plants under’low light’,’medium light’and’high light’were significantly higher than those under’control group’,indicating that the photosynthetic physiological function of plants under’control group’was weak.The fast light response curve test showed that the electron transfer rate(rETR)increased rapidly with the increase of PAR at four light intensities,but the maximum electron transfer efficiency(rETRmax),half-full light intensity(Ik)and initial slope(α)all showed the decreasing trend of’low light’>’medium light’>’high light’>’control group’,which meant the photochemical efficiency of‘low light’was stronger.The highest effective photochemical efficiency and actual photochemical efficiency under four light intensity conditions were‘low light’.

关 键 词：反瓣虾脊兰 叶绿素荧光参数 光照条件 光化学效率 

分 类 号：S718.5[农业科学—林学] Q945.11[生物学—植物学]