抗弓形虫和新孢子虫药物体外筛选方法的研究进展  

Recent Advances in Screening Technology of Drugs against Toxoplasma gondii and Neospora caninum In Vitro

作　　者：刘海燕 李小瑾 严文岚 赵立卓 王天奇[1] 钱伟锋[1] LIU Hai-yan;LI Xiao-jin;YAN Wen-lan;ZHAO Li-zhuo;WANG Tian-qi;QIAN Wei-feng(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang471003,China)

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003

出　　处：《畜牧与饲料科学》2023年第1期122-128,共7页Animal Husbandry and Feed Science

基　　金：河南省高等学校重点科研项目(20A230001)。

摘　　要：弓形虫和新孢子虫是2种在形态结构及生物学特征方面非常相似的细胞内寄生性原虫,二者引起的疾病对人和动物健康造成了严重危害。开发和筛选高效、低毒的抗弓形虫和新孢子虫药物一直是抗原虫药物研究领域的重要方向之一。药物筛选包括体外筛选和体内筛选,体外筛选的结果是进行体内筛选的依据和基础。对抗弓形虫和新孢子虫药物的体外筛选方法研究进展进行综述,以期为开发更加高效的抗弓形虫和新孢子虫药物体外筛选技术提供参考。Toxoplasma gondii(T.gondii)and Neospora caninum(N.caninum)are intracellular parasitic protozoa with very similar morphological structure and biological characteristics.The diseases caused by these parasites pose serious harm to human and animal health.Development and screening of high-efficient and low-toxic drugs against T.gondii and N.caninum has long been one of the important directions in the research field of antiprotozoal drugs.Drug screening includes the experiments in vitro and in vivo,and the foundings of screening in vitro are the basis and foundation for selection in vivo.The recent advances in screening technology of drugs against T.gondii and N.caninum in vitro are reviewed,in hoping to provide references for the development of more efficient screening technology of drugs against these parasites in vitro.

关 键 词：弓形虫 新孢子虫 药物筛选方法 转基因虫体 qPCR 

分 类 号：S855.9[农业科学—临床兽医学] S859.795[农业科学—兽医学]