苯硼酸聚合物刷微图案的制备及响应性能  

Fabrication and Stimuli-responsiveness of Phenylboronic Acid-containing Polymer Brush Micropattern

作　　者：赵海利 魏中华 沙金[2] 谢林生[2] 马玉录[2] 陈涛 Hai-li Zhao;Zhong-hua Wei;Jin Sha;Lin-sheng Xie;Yu-lu Ma;Tao Chen(Faculty of Chemical Engineering,Kunming University of Science and Technology,Kunming 650504;School of Mechanical and Power Engineering,East China University of Science and Technology,Shanghai 200237)

机构地区：[1]昆明理工大学化学工程学院,昆明650504 [2]华东理工大学机械与动力工程学院,上海200237

出　　处：《高分子学报》2023年第2期257-265,共9页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号52103262);云南省科技厅“放管服”及稳定支持专项(项目号202001BB050007)资助.

摘　　要：以3-甲基丙烯酰胺基苯硼酸(MAPBA)为聚合反应单体,通过数字微镜器件(DMD)调控光辐照引发表面原子转移自由基聚合(ATRP)反应制备苯硼酸(PMAPBA)聚合物刷微图案.采用光学显微镜、X射线光电子能谱测试(XPS)和飞行时间二次离子质谱测试(TOF-SIMS)对所制备微图案的几何形状、化学组成及分布进行表征,结果表明PMAPBA聚合物刷微图案在硅基体表面的成功制备.研究了PMAPBA聚合物刷微图案的pH和葡萄糖响应性并采用激光共聚焦显微镜对其结果进行表征分析,结果表明随着溶液pH值的升高,苯硼酸发生电离产生带负电的亲水离子会阻碍免疫球蛋白(IgG)而促进葡聚糖(dextran)在其表面的吸附;此外,当溶液中加入葡萄糖后,电离产生的亲水离子不断与葡萄糖结合而导致IgG分子的脱落.这种具有pH和葡萄糖双重响应性的PMAPBA聚合物刷图案化表面能够为动态生物活性表面和药物可控释放系统的制备提供新的途径.The smart biointerface with the capability to dynamically control the interaction between the biomolecules and material interface has attracted much attention.Here,a responsive smart biointerface was created based on the boronic acid-containing polymer brush micropattern.With 3-methacrylamidophenylboronic acid as monomer,micropatterned poly(3-methacrylamidophenylboronic acid)(PMAPBA)brushes were fabricated through combining the digital mirror device(DMD)-based light modulation technique and surfaceinitiated photoinduced atom transfer radical polymerization(Photo-ATRP).A series of methods such as optical microscopy,X-ray photoelectron spectroscopy,and time of flight-secondary ion mass spectrometry were applied to characterize the geometric shape,chemical composition and distribution of the resulting brush structure.The pH-responsiveness of micropatterned PMAPBA brushes was investigated with fluorescein isothiocyanate stained immunoglobulin G(IgG),and the result from laser confocal microscope revealed that the increase in the pH of the solution will hinder the immobilization of IgG protein on the PMAPBA brushes,which was attributable to the negatively charged hydrophilic ions produced by the ionization of phenylboronic acid.Besides,the catecholboronate interaction between phenylboronic acid moieties and the secondary hydroxyls on the dextran could promote the immobilization of dextran on the PMAPBA brushes under neutral and alkaline conditions.In addition,the sugar-responsiveness of PMAPBA brushes enabled dynamic modulation of IgG adsorption behavior.The PMAPBA brushes micropatterned surface with dual stimuli-responsive could provide a novel route for the preparation of dynamic bioactive surfaces and controlled drug release systems.

关 键 词：苯硼酸 聚合物刷微图案 PH响应 糖响应 

分 类 号：TQ317[化学工程—高聚物工业]