59个Y-STR基因座的法医学多态性应用效能评估  

Efficacy Evaluation of Forensic Polymorphism Application of 59 Y-STR

作　　者：刘大宇 徐际超 曾滢 徐曲毅[2] 刘宏[2] 李越[2] 刘长晖[2] LIU Dayu;XU Jichao;ZENG Ying;XU Quyi;LIU Hong;LI Yue;LIU Changhui(Huangpu Branch of Guangzhou Public Security Bureau,Guangzhou 510700,China;Guangzhou Institute of Forensic Science,Guangzhou 510442,China;School of Forensic Medicine,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]广州市公安局黄埔区分局,广州510700 [2]广州市刑事科学技术研究所,广州510442 [3]南方医科大学法医学院,广州510515

出　　处：《生物技术进展》2023年第1期107-114,共8页Current Biotechnology

基　　金：广州市科技计划项目(2019030014;2019030016)。

摘　　要：为了评估法医学DNA数据库建设中涉及的59个Y-STR基因座的遗传多态性和法医学应用效能,通过AGCU Y SUPP PLUS试剂盒和AGCU Y37试剂盒检测374个广东汉族无关男性个体,将检测出的59个Y-STR基因座按照突变率的高低进行分类组合和统计分析。结果显示,59个Y-STR基因座联合运用在374个无关男性个体中检出了374个单倍型,其中44个中低突变Y-STR组合、15个高快突变Y-STR组合分别检出373和372个单倍型。59个Y-STR的基因多态性数值分布在0.0551(DYS645)~0.9580(DYF387S1 a/b)之间。结果表明,这59个Y-STR在广东汉族群体中均具有良好的多态性,按中低突变Y-STR组合和高快突变Y-STR组合研发新的检测体系可更好地满足法医实践的不同需求。To evaluate the polymorphism of 59 Y-STR loci,being involved into forensic DNA database construction,a total of 374 unrelated Guangdong Han-ethnic male individuals were genotyped with both AGCU Y SUPP PLUS and AGCU Y37 kits by their different combinations,along with the resultant forensic efficiency appraised.The efficiency of 59 Y-STRs was estimated according to different genetic categories:high-and-rapid and/or slow-and-moderate mutating Y-STR combinations.The results showed that of the 374 Guangdong Han-ethnic unrelated male individuals,374 haplotypes were detected by all the Y-STR 59-locus combination,with 372 and 373 ones being discovered from the high-and-rapid mutating 15-locus Y-STRs and slow-and-moderate 44-locus Y-STRs,respectively.The values of gene diversity ranged from 0.0551(DYS645)to 0.9580(DYF387S1 a/b).Both two kits are of great polymorphism in Guangdong Han-ethnic population,suggesting that the various forensic requirements will be better met if novel testing systems are to develop based on the combination of slow-and-moderate and/or high-and-rapid mutating Y-STRs.

关 键 词：法医遗传学 Y-STR 中低突变Y-STR 高快突变Y-STR 单倍型 

分 类 号：DF795.2[医药卫生—法医学]