核磁共振定量碳谱测定食用油脂肪酸组成综合实验设计  

Comprehensive Experimental Design for Determination of Fatty Acid Composition in Edible Oil by ^(13)C-NMR Method

作　　者：邱千 廖晓燕[1] 邱国福[1] QIU Qian;LIAO Xiaoyan;QIU Guofu(School of Pharmaceutical Sciences,Wuhan University,Wuhan 430071,China;Department of Pharmacy,The Central Hospital of Wuhan,Wuhan 430014,China)

机构地区：[1]武汉大学药学院,武汉430071 [2]武汉市中心医院药学部,武汉430014

出　　处：《实验科学与技术》2022年第6期120-125,共6页Experiment Science and Technology

基　　金：湖北高校省级教学研究项目(202040);武汉大学教学研究重点项目(2019JG017);武汉大学教学改革研究项目(JG201670)。

摘　　要：核磁共振波谱仪是药学专业重要的分析仪器,是药学领域强有力的定性分析工具之一。然而核磁共振波谱仪同样可用于定量分析,因此设计了不需要任何前处理和高温过程,且方法稳定、重现性好的核磁共振定量碳谱测定食用油脂肪酸主要组成与相对含量的综合实验。利用反门控去耦碳谱测试实验,通过比对找出各脂肪酸的特征信号峰,并以此定性判断食用油中各脂肪酸特别是不饱和脂肪酸的组成,同时通过对特征信号峰积分测定各脂肪酸的含量,使学生能够深刻掌握核磁共振波谱仪的定性与定量功能。NMR is one of the most powerful qualitative analysis tools in the field of pharmacy.However,NMR can also be used for quantitative analysis,so a comprehensive experiment is designed to determine the main composition and relative content of fatty acids in edible oil by quantitative ^(13)C-NMR without any pretreatment and high temperature process,with stable method and good reproducibility.By using the reverse gating decoupling carbon spectroscopy test experiment,the characteristic signal peaks of each fatty acid are found,and the composition of fatty acids,especially unsaturated fatty acids is qualitative analyzed.The content of fatty acids determined by the integration of characteristic signal peaks.Students could deeply master the qualitative and quantitative functions of NMR though the comprehensive experiment.

关 键 词：核磁共振 定量碳谱 脂肪酸 反门控去耦 

分 类 号：R284.1[医药卫生—中药学]