人血中6种苯系物测定的吹扫捕集-气相色谱质谱法  

Determination of six benzene homologues in human blood by purge and trap-gas chromatography-mass spectrometry

作　　者：赖璟琦[1] 邓朗静 冯奋栋[1] 赖少阳[1] 叶小莉 Lai Jingqi;Deng Langjing;Feng Fendong;Lai Shaoyang;Ye Xiaoli(Laboratory of Physical and Chemical,Shenzhen Luohu District Center for Disease Control and Prevention,Shenzhen 518020,China;Public Health,School of Public Health,Jinan University,Guangzhou 510632,China)

机构地区：[1]深圳市罗湖区疾病预防控制中心理化检验科,深圳518020 [2]暨南大学公共卫生学院公共卫生专业,广州510632

出　　处：《中华劳动卫生职业病杂志》2022年第11期857-860,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：2019年罗湖区软科学研究计划项目(LX20190501)。

摘　　要：目的建立基于土壤分析模式的吹扫捕集-气相色谱质谱法测定人血中苯、甲苯、乙苯、间二甲苯、对二甲苯和邻二甲苯6种苯系物。方法于2020年9月至2021年5月,以硅藻土为分散剂加至2.0 ml血样中充分混匀,直接在吹扫捕集瓶内进行吹扫后进样,气相色谱柱分离,采用保留时间锁定定性,选择离子扫描模式(SIM)检测,并对方法的检出限和回收率等指标进行分析。结果人血中6种苯系物测定方法的线性范围为0.02~10.00 ng/ml,相关系数0.9927~0.9968,方法检出限0.006~0.016 ng/ml,样品加标回收率84.39%~102.41%,方法精密度3.06%~6.90%。结论吹扫捕集-气相色谱质谱法可同时测定人血中6种苯系物的含量,前处理方法简单省时,检出限低,为人体苯系物内暴露检测方法提供科学依据。Objective To establish a purge and trap-gas chromatography-mass spectrometry method based on soil analysis model for the determination of six benzene homologues(benzene,toluene,ethylbenzene,m-xylene,p-xylene and o-xylene)in human blood.Methods From September 2020 to May 2021,diatomite was used as a dispersant to add 2.0 ml blood sample and fully mixed.The sample was directly injected into the purging and collecting bottle after purging.The gas chromatography column was used for separation.The retention time locking was used for qualitative analysis and the selected ion scanning mode(SIM)was used for detection.The detection limit and recovery rate of the method were analyzed.Results The linear range of the method for the determination of six benzene homologues in human blood was 0.02-10.00 ng/ml,the correlation coefficient was 0.9927-0.9968,the detection limit was 0.006-0.016 ng/ml,the recovery rate of sample spiking was 84.39%-102.41%,and the precision of the method was 3.06%-6.90%.Conclusion Purge and trap-gas chromatography-mass spectrometry can simultaneously determine the contents of six benzene homologues in human blood.The pretreatment method is simple,time-saving,and the method has low detection limit,which provides a scientific basis for the detection of benzene homologues in human body.

关 键 词：气相色谱-质谱法 苯系物 吹扫捕集 血 

分 类 号：O657.63[理学—分析化学] R446.11[理学—化学]