Regulation of floral senescence in Arabidopsis by coordinated action of CONSTANS and jasmonate signaling  

作　　者：Gloria Serrano-Bueno Pedro de los Reyes Andrea Chini Gabriel Ferreras-Garrucho Víctor Sánchez de Medina-Hernández Marta Boter Roberto Solano Federico Valverde 

机构地区：[1]Plant Development Group,Institute for Plant Biochemistry and Photosynthesis,CSIC-Universidad de Sevilla,41092 Sevilla,Spain [2]Department of Plant Molecular Genetics,Centro Nacional de Biotecnología,CSIC,28049 Madrid,Spain [3]Crop Science Centre,University of Cambridge,Cambridge CB3 OLE Cambridge,UK [4]Gregor Mendel Institute(GMI),Austrian Academy of Sciences,Vienna BioCenter(VBC),Vienna,Austria

出　　处：《Molecular Plant》2022年第11期1710-1724,共15页分子植物（英文版）

基　　金：Authors would like to acknowledge the help or Drs.M,Calonje.And D.Pozo,and prof.M.A,Blazquez for critical reading of the manuscript;Work by G.S.-B.was supported by a European.Union.contract LONGFLOW(MSCAIF-2018-838317);CSICLONGFLOW_(CON-V_EXT_014);Wealso acknowledge financial support of from the Spanish Ministry for Science and Innovations(MICINN/FEDER)grants BIO2017-84066-R(to.F.V.),PPID2020-117018RB-100(to F.V.),and PID2019-107012RB-100(to R.S.and A.C.).

摘　　要：In Arabidopsis,photoperiodic flowering is controlled by the regulatory hub gene CONSTANS(CO),whereas floral organ senescence is regulated by the jasmonates(JAs).Because these processes are chronologically ordered,it remains unknown whether there are common regulators of both processes.In this study,we discovered that CO protein accumulates in Arabidopsis flowers after floral induction,and it displays a diurnal pattern in floral organs different from that in the leaves.We observed that altered CO expression could affect flower senescence and abscission by interfering with JA response,as shown by petal-specific transcriptomic analysis as well as CO overexpression in JA synthesis and signaling mutants.We found that CO has a ZIM(ZINC-FINGER INFLORESCENCE MERISTEM)like domain that mediates its interaction with the JA response repressor JAZ3(jasmonate ZIM-domain 3).Their interaction inhibits the repressor activity of JAZ3,resulting in activation of downstream transcription factors involved in promoting flower senescence.Furthermore,we showed that CO,JAZ3,and the E3 ubiquitin ligase COI1(Coronatine Insensitive 1)could form a protein complex in planta,which promotes the degradation of both CO and JAZ3 in the presence of JAs.Taken together,our results indicate that CO,a key regulator of photoperiodic flowering,is also involved in promoting flower senescence and abscission by augmenting JA signaling and response.We propose that coordinated recruitment of photoperiodic and JA signaling pathways could be an efficient way for plants to chronologically order floral processes and ensure the success of offspring production.

关 键 词：CONSTANS flower senescence jasmonic acid 

分 类 号：Q94[生物学—植物学]