Activation tagging identifies WRKY14 as a repressor of plant thermomorphogenesis in Arabidopsis  

作　　者：Wenqi Qin Ning Wang Qi Yin Huiling Li Ai-Min Wu Genji Qin 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University,Guangzhou 510642,China [2]State Key Laboratory of Protein and Plant Gene Research,School of Life Sciences,Peking University,Beijing 100871,People’s Republic of China [3]These authors contributed equally to this article.Affiliations

出　　处：《Molecular Plant》2022年第11期1725-1743,共19页分子植物（英文版）

基　　金：National Science Fund for Distinguished Young Scholars of China(grant no.31725005);National Natural Science Foundation of China(grant no.31970194);National Key Research and Development Program of China(2017YFA0503800).

摘　　要：Increases in recorded high temperatures around the world are causing plant thermomorphogenesis and decreasing crop productivity.PHYTOCHROME INTERACTING FACTOR 4(PIF4)is a central positive regulator of plant thermomorphogenesis.However,the molecular mechanisms underlying PIF4-regulated thermomorphogenesis remain largely unclear.In this study,we identified ABNORMAL THERMOMORPHOGENESIS 1(ABT1)as an important negative regulator of PIF4 and plant thermomorphogenesis.Overexpression of ABT1 in the activation tagging mutant abt1-D caused shorter hypocotyls and petioles under moderately high temperature(HT).ABT1 encodes WRKY14,which belongs to subgroup II of the WRKY transcription factors.Overexpression of ABT1/WRKY14 or its close homologs,including ABT2/WRKY35,ABT3/WRKY65,and ABT4/WRKY69in transgenic plants caused insensitivity to HT,whereas the quadruple mutant abt1 abt2 abt3 abt4 exhibited greater sensitivity to HT.ABTs were expressed in hypocotyls,cotyledons,shoot apical meristems,and leaves,but their expression were suppressed by HT.Biochemical assays showed that ABT1 can interact with TCP5,a known positive regulator of PIF4,and interrupt the formation of the TCP5-PIF4 complex and repress its transcriptional activation activity.Genetic analysis showed that ABT1 functioned antagonistically with TCP5,BZR1,and PIF4 in plant thermomorphogenesis.Taken together,our results identify ABT1/WRKY14 as a critical repressor of plant thermomorphogenesis and suggest that ABT1/WRKY14,TCP5,and PIF4 may form a sophisticated regulatory module to fine-tune PIF4 activity and temperature-dependent plant growth.

关 键 词：plant thermomorphogenesis WRKY transcription factor TCP transcription factor ABT1 PIF4 

分 类 号：Q94[生物学—植物学]