Pepper variome reveals the history and key loci associated with fruit domestication and diversification  

作　　者：Yacong Cao Kang Zhang Hailong Yu Shumin Chen Donghui Xu Hong Zhao Zhenghai Zhang Yinqing Yang Xiaozhen Gu Xinyan Liu Haiping Wang Yaxin Jing Yajie Mei Xiang Wang Véronique Lefebvre Weili Zhang Yuan Jin Dongliang An Risheng Wang Paul Bosland Xixiang Li IIan Paran Baoxi Zhang Giovanni Giuliano Lihao Wang Feng Cheng 

机构地区：[1]Key Laboratory of Vegetables,Genetics,and Physiology of China Ministry of Agriculture and Rural Affairs,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs,Sino-Dutch Joint Laboratory of Horticultural Genomics,Institute of Vegetables and Flowers,CAAS(Chinese Academy of Agricultural Sciences),12 Zhongguancun South Street,Beijing 100081,P.R.China [2]INRAE,GAFL,Unitéde Génétique et Amélioration des Fruits et Légumes,84140 Montfavet,France [3]Institute of Vegetables,Academy of Agricultural Sciences of Guangxi,174 Daxue East Road,Nanning 53007,P.R.China [4]Department of Plant and Environmental Sciences,NMSU,Las Cruces,NM 88003,USA [5]Institute of Plant Sciences,Agricultural Research Organization,The Volcani Center,Rishon LeZion,Israel [6]Biotechnology and Agroindustry Division,ENEA,Italian National Agency for New Technologies,Energy and Sustainable Development,Via Anguillarese,301-00123 Roma,Italy

出　　处：《Molecular Plant》2022年第11期1744-1758,共15页分子植物（英文版）

基　　金：China National Key Technology Research and Development Program(2016YFD0100200,2016YFD0101700,2018YFD1000800,and 2020YFD1001100);National Natural Science Foundation of China(NSFC grants 31722048,31972411,3170110879,32102382,and 32102386);Central Public-interest Scientific Institution Basal Research Fund(Y2022GH04,IVF-BRF2021015);Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS);Earmarked Fund for the China Agriculture Research System(CARS-25);CAAS-GAAS Coordinated Innovation Project of the Chinese Academy of Agricultural Sciences(2019);Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture and Rural Affairs,P.R.China;Horizon 2020 G2P-SOL project(grant no.677379)from the EU.

摘　　要：Pepper(Capsicum spp.)is an important vegetable crop that provides a unique pungent sensation when eaten.Through construction of a pepper variome map,we examined the main groups that emerged during domestication and breeding of C.annuum,their relationships and temporal succession,and the molecular events underlying the main transitions.The results showed that the initial differentiation in fruit shape and pungency,increase in fruit weight,and transition from erect to pendent fruits,as well as the recent appearance of large,blocky,sweet fruits(bell peppers),were accompanied by strong selection/fixation of key alleles and introgressions in two large genomic regions.Furthermore,we identified Up,which encodes a BIG GRAIN protein involved in auxin transport,as a key domestication gene that controls erect vs pendent fruit orientation.The up mutation gained increased expression especially in the fruit pedicel through a 579-bp sequence deletion in its 5′upstream region,resulting in the phenotype of pendent fruit.The function of Up was confirmed by virus-induced gene silencing.Taken together,these findings constitute a cornerstone for understanding the domestication and differentiation of a key horticultural crop.

关 键 词：CAPSICUM variome DOMESTICATION fruit orientation PUNGENCY fruit shape sweet pepper blocky fruit pepper narrow fruit pepper 

分 类 号：S641.3[农业科学—蔬菜学]