Cell-specific pathways recruited for symbiotic nodulation in the Medicago truncatula legume  

作　　者：Sergio Alan Cervantes-Pérez Sandra Thibivilliers Carole Laffont Andrew D.Farmer Florian Frugier Marc Libault 

机构地区：[1]Department of Agronomy and Horticulture,Center for Plant Science Innovation,University of Nebraska-Lincoln,Lincoln,NE 68503,USA [2]Single Cell Genomics Core Facility,Center for Biotechnology,University of Nebraska-Lincoln,Lincoln,NE 68588,USA [3]Institute of Plant Sciences Paris-Saclay(IPS2),UniversitéParis-Saclay,CNRS,INRAE,UniversitéParis-Cité,Universitéd’Evry,91190 Gif-sur-Yvette,France [4]National Center for Genome Resources,Santa Fe,NM 87505,USA

出　　处：《Molecular Plant》2022年第12期1868-1888,共21页分子植物（英文版）

基　　金：Supported by grants to M.L.from the U.S.National Sclence Foundation (I0S#1854326 and 2127485),USDA-NIFA(2022-67013-36144);by the Center for Plant Science Innovation,and by the Department of Agronomy and Horticulture at the University of Nebraska-Lincoln.Work in F.F.labo-ratory was supported by the"Ecole Universitaire de Recherche"Saclay Plant Sciences(EUR-SPS).

摘　　要：Medicago truncatula is a model legume species that has been studied for decades to understand the symbiotic relationship between legumes and soil bacteria collectively named rhizobia.This symbiosis called nodulation is initiated in roots with the infection of root hair cells by the bacteria,as well as the initiation of nodule primordia from root cortical,endodermal,and pericycle cells,leading to the development of a new root organ,the nodule,where bacteria fix and assimilate the atmospheric dinitrogen for the benefit of the plant.Here,we report the isolation and use of the nuclei from mock and rhizobia-inoculated roots for the single nuclei RNA-seq(sNucRNA-seq)profiling to gain a deeper understanding of early responses to rhizobial infection in Medicago roots.A gene expression map of the Medicago root was generated,comprising 25 clusters,which were annotated as specific cell types using 119 Medicago marker genes and orthologs to Arabidopsis cell-type marker genes.A focus on root hair,cortex,endodermis,and pericycle cell types,showing the strongest differential regulation in response to a short-term(48 h)rhizobium inoculation,revealed not only known genes and functional pathways,validating the sNucRNA-seq approach,but also numerous novel genes and pathways,allowing a comprehensive analysis of early root symbiotic responses at a cell type-specific level.

关 键 词：Medicago root single-cell transcriptomic RHIZOBIUM nodule initiation root hair cells cortical cells 

分 类 号：Q943.2[生物学—植物学]