G protein controls stress readiness by modulating transcriptional and metabolic homeostasis in Arabidopsis thaliana and Marchantia polymorpha  

作　　者：Ting-Ying Wu Shalini Krishnamoorthi Kulaporn Boonyaves Isam Al-Darabsah Richalynn Leong Alan M.Jones Kimitsune Ishizaki Kang-Ling Liao Daisuke Urano 

机构地区：[1]Temasek Life Sciences Laboratory,Singapore,Singapore [2]Department of Mathematics,University of Manitoba,Winnipeg,MB,Canada [3]Singapore-MIT Alliance for Research and Technology,Singapore,Singapore [4]Department of Biological Sciences,National University of Singapore,Singapore 117558,Singapore [5]Departments of Biology and Pharmacology,University of North Carolina,Chapel Hill,NC,USA [6]Graduate School of Science,Kobe University,Kobe,Hyogo 657-8501,Japan

出　　处：《Molecular Plant》2022年第12期1889-1907,共19页分子植物（英文版）

基　　金：This study was supported by the Agency for Science,Technology and Research(A*STAR)Singapore under the Industry Alignment Fund Pre-positioning Program,the High Performance Precision Agriculture(HiPPA)system(A19E4a0101);the Singapore-MIT Aliance for Research and Technology,and Disruptive&Sustainable Technologies for Agricul-tural Precision(DISTAP)(to D.U.);a discovery grant from the Natural Sciences and Engineering Research Council of Canada(ARGPIN-2020-07097)(to K-LL)。

摘　　要：The core G protein signaling module,which consists of Gαand extra-large Gα(XLG)subunits coupled with the Gβγdimer,is a master regulator of various stress responses.In this study,we compared the basal and salt stress-induced transcriptomic,metabolomic and phenotypic profiles in Gα,Gβ,and XLG-null mutants of two plant species,Arabidopsis thaliana and Marchantia polymorpha,and showed that G protein mediates the shift of transcriptional and metabolic homeostasis to stress readiness status.We demonstrated that such stress readiness serves as an intrinsic protection mechanism against further stressors through enhancing the phenylpropanoid pathway and abscisic acid responses.Furthermore,WRKY transcription factors were identified as key intermediates of G protein-mediated homeostatic shifts.Statistical and mathematical model comparisons between A.thaliana and M.polymorpha revealed evolutionary conservation of transcriptional and metabolic networks over land plant evolution,whereas divergence has occurred in the function of plant-specific atypical XLG subunit.Taken together,our results indicate that the shifts in transcriptional and metabolic homeostasis at least partially act as the mechanisms of G protein-coupled stress responses that are conserved between two distantly related plants.

关 键 词：homeostatic adjustments transcriptional regulation metabolic regulation heterotrimeric G protein evolutionary conservation 

分 类 号：Q94[生物学—植物学]