HPLC法测定固定化青霉素酰化酶合成头孢克洛的活力  

Measurement of the Activity of the Synthesized Cefaclor from Immobilized Penicillin Acylase by HPLC

作　　者：刘萍 王利杰 田洪年 任峰 张建丽 李晓景 郭自然 石春利 LIU Ping;WANG Li-je;TIAN Hong-nian;REN Feng;ZHANG Jian-li;LI Xiao-jing;GUO Ziran;SHI Chun-li(NCPC Hebei Huamin Pharmaceutical Co.,Ltd.,Shijiazhuang 052165,China;National and Local Joint Engineering Laboratory of Antibiotic Enzyme Catalysis and Crystallization Technology,Shijiazhuang 052165,China;Hebei Province Cephalosporide Medicine Preparation Technology Innovation Center,Shijiazhuang 052165,China;Hebei Lansheng Biotech Co.,Ltd.,Jinzhou 052260,China)

机构地区：[1]华北制药河北华民药业有限责任公司,河北石家庄052165 [2]抗生素酶催化与结晶技术国家地方联合工程实验室,河北石家庄052165 [3]河北省头孢类药物制备技术创新中心,河北石家庄052165 [4]河北兰升生物科技有限公司,河北晋州052260

出　　处：《精细化工中间体》2022年第6期90-93,共4页Fine Chemical Intermediates

摘　　要：建立了一种利用高效液相色谱法(HPLC)测定固定化青霉素酰化酶合成头孢克洛活力的方法,经过验证,头孢克洛在2.879~8.638μg/mL范围内线性关系良好(r=1.0000),回收率在99%~101%范围内,RSD为0.43%。该方法操作简便,专属性强,结果可靠,可用于测定固定化青霉素酰化酶合成头孢克洛的活力。A HPLC method was established for the determination of the activity of immobilized penicillin acylase in the synthesis of Cefaclor.The linearity of calibration curve for Cefaclor was good in the range of2.879 to 8.638μg/mL(r was 1.0000);the recoveries were in the range of 99% to 101%;and the RSD was0.43%.This established method is simple,specific and reliable,which can be used to determine the activity of immobilized penicillin acylase in the synthesis of Cefaclor.

关 键 词：高效液相色谱法 固定化青霉素酰化酶 头孢克洛 活力 

分 类 号：R978.1[医药卫生—药品] R917[医药卫生—药学]